重组人胰岛素样生长因子I(rhIGF-I)对大鼠成骨细胞增殖和功能的影响  

The effect of recombinant human insulin-like growth factor (rhIGF-I) on the proliferation and anabolic function of cultured rat osteoblasts

作　　者：徐萍[1] 张克勤[1] 刘超[1] 刘翠萍[1] 唐伟[1] 

机构地区：[1]南京医科大学第一附属医院内分泌科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2005年第7期472-475,F002,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:观察胰岛素样生长因子I(IGF鄄I)对体外培养的大鼠成骨细胞的增殖、碱性磷酸酶(ALP)的合成和钙化结节形成的影响。方法:不同浓度IGF鄄I分别刺激培养的大鼠成骨细胞,采用噻唑蓝(MTT)法测定细胞增殖能力;采用对硝基酚磷酸盐法测定细胞裂解液中ALP活性;VonKossa染色测定钙化结节数目。结果:一定浓度IGF鄄I能明显增加大鼠成骨细胞数量(P<0.05),在0.1～100.0ng/ml这种作用与IGF鄄I的浓度呈正相关;经IGF鄄I刺激,大鼠成骨细胞ALP合成明显高于对照组(P<0.05);经IGF鄄I刺激,大鼠成骨细胞钙化结节数目明显高于对照组(P<0.001)。结论:IGF鄄I能增加体外培养的大鼠成骨细胞数量,促进成骨细胞ALP合成和钙化,提示IGF鄄I可能直接促进骨形成。Objective: To investigate the effect of rhIGF-I on the proliferation and anabolic function of culture rat osteoblasts in vitro. Methods: Rat calvaria cells were cultured in medium with or without IGF-I. MTT assay was used to assess the cell number; PNPP method was used to measure the ALP content within osteoblasts; the calcium nodules in the culture dishes were quantitated based on Von Kossa's staining. Results: The cell number in osteoblasts exposed to IGF-I at 0.1-100.0 ng/ml increased significantly compared with that in osteoblasts exposed to vehicle medium(P < 0.05). The ALP activity in IGF-I-treated cells was higher than that in vehicle-treated cells(P < 0.05). The calcium nodule number was greater in IGF-I-treated cells than that in vehicle-treated cells(P < 0.001). Conclusion: IGF-I can increase the osteoblastic cell number and promote the calcification of extracellular matrix in vitro. These results suggest that IGF-I probably promote bone formation in vitro.

关 键 词：胰岛素样生长因子Ⅰ 成骨细胞 增殖 碱性磷酸酶 钙化结节 

分 类 号：R587.1[医药卫生—内分泌]