植物多糖对S180、K562细胞增殖和唾液酸、磷脂、胆固醇含量的影响  

Effects of Plant Polysaccharides on Cell Proliferation and Cell Membrane Contents of Sialic Acid,Phospholipid and Cholesterol in S 180 and K 562 Cells

作　　者：佟丽[1] 黄添友[1] 李吉来[1] 吴波[1] 梁谋[1] 梁念慈[1] 

机构地区：[1]第一军医大学中西医结合研究所,广东医学院医用生物化学研究所

出　　处：《中国中西医结合杂志》1994年第8期482-484,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：全军青年科学基金

摘　　要：本实验用ＭＴＴ比色分析法研究了茯苓多糖（ＰＰＳ）、刺五加多糖（ＡＳＰＳ）、银耳多糖（ＴＦ）和香菇多糖（Ｌｅｎｔｉｎａｎ）４种植物多糖对体外培养的小鼠腹水型肉瘤Ｓ１８０细胞和人慢性骨髓性白血病Ｋ５６２细胞增殖的影响，结果证实ＰＰＳ、ＡＳＰＳ在体外具有直接抗肿瘤作用。ＰＰＳ对两种肿瘤细胞的半数有效抑制浓度（ＩＣ５０）均为１．５ｍｇ／ｍｌ，ＡＳＰＳ对Ｓ１８０和Ｋ５６２细胞的ＩＣ５０分别为０．３８ｍｇ／ｍｌ和０．２８ｍｇ／ｍｌ；ＴＦ和Ｌｅｎｔｉｎａｎ对两种细胞的增殖无抑制作用。本实验同时检测了ＰＰＳ、ＡＳＰＳ作用于Ｓ１８０细胞２４ｈ后，细胞膜唾液酸（ＳＡ）、磷脂（Ｐｌ）、胆固醇（Ｃｈ）含量的变化，发现除Ｃｈ及Ｃｈ／Ｐｌ比值无明显变化外，ＳＡ升高（Ｐ＜０．０５）、Ｐｌ减少（Ｐ＜０．０５），提示ＰＰＳ、ＡＳＰＳ的抗肿瘤作用可能与之有关。The four kinds of plant polysaccharides,i.e.,pachyman polysaccharides(PPS),Acanthopanax senticosus polysaccharides(ASPS) ,polysaccharides of tremella fuciformis(TF) and lentinan,have obviously inhibitory action against the animal tumor growth and have been applied to the treatment of cancer. The mechanism was that they could enhance the body immune function, but whether the tumor cells were killed is not clear. In this paper, the effects of the four plant polysaccharides on cell proliferation in mice sarcoma (ascitic type) S180 and human chronic myelogenous leukemia K562 cells were studied with MTT chromometry. Tt was found That TF and lentinan had no effect on both cell line,but PPS and ASPS could obviously inhibit the proliferation of them,the IC50 of PPS was 1.5mg/ml in both cell line,that of ASPS was 0.38 mg/ml(S180 cells) and 0.28mg/ml (K562 cells)respectively.This result indicated that the PPS and ASPS were able to kill the tumor cell directly.To investigate the mechanism of antitumor action of PPS and ASPS, the sialic acid (SA),phospholipid(PI) and cholesterol (Ch) contents of S180 cell membrane were examined after the PPS or ASPS application for 24 hours. No significant changes were observed for the Ch and Ch/pl ratio,the amount of SA increased and that of PI lowered respectively(P<0.05).The results suggested that the antitumor action of PPS and ASPS not only related to the action of enhancing the body immune function but also related to the changes of cell membrane.

关 键 词：植物多糖 肿瘤 细胞增殖 唾液酸 

分 类 号：R979.1[医药卫生—药品]