葡甘聚糖对四氧嘧啶糖尿病模型大鼠的疗效观察  

Observation on the therapeutic effect of Glucomannan in diabetes through animal experimentation

作　　者：王小燕[1] 顾耀[2] 闵旸 盛雨辰 

机构地区：[1]上海市医学会,200040 [2]同济大学附属上海市第十人民医院 [3]上海医药工业研究所

出　　处：《上海医学》2005年第5期406-411,共6页Shanghai Medical Journal

摘　　要：目的观察葡甘聚糖对正常大鼠和糖尿病大鼠的降血糖效果,测定糖尿病大鼠α-葡萄糖苷酶(α-糖苷酶)水平,初步探讨葡甘聚糖的作用机制,为临床应用提供理论依据。方法①将SD大鼠分为6组:正常组:给予生理盐水;阴性组:分别给予蔗糖、葡萄糖;阳性组:分别给予蔗糖、葡萄糖和伏格列波糖0.043mg·ml-1·100g体重-1;低剂量组:分别给予蔗糖、葡萄糖和葡甘聚糖0.05g·ml-1·100g体重-1;中剂量组:分别给予蔗糖、葡萄糖和葡甘聚糖0.1g·ml-1·100g体重-1;高剂量组:分别给予蔗糖、葡萄糖和葡甘聚糖0.5g·ml-1·100g体重-1。②雄性SD大鼠禁食24h后,腹腔注射20mg·ml-1·100g体重-1的四氧嘧啶水溶液,72h后从尾静脉取血,挑选出血糖浓度>10mmol/L的大鼠,随机分为5组:阴性组、阳性组分别给予生理盐水和上述剂量的伏格列波糖,低、中和高剂量组分别给予上述剂量的葡甘聚糖。③采用体内、体外实验测定大鼠的α糖苷酶活性。结果①对正常大鼠蔗糖耐量作用:阳性组与正常组血糖升高面积(△糖面积)的差异无显著性,均显著低于阴性组(P<0.05)。低、中和高剂量组的△糖面积显著高于阳性组(P<0.05),分别为阳性组的3.3、3.0和2.7倍,但仍显著低于阴性组(P<0.05)。低、中、高剂量组间的差异无显著性(P>0.05)。②对正常大鼠葡萄糖耐量作用:与?Objective To explore the possible mechanism of glucomannan by investigating the antihyperglycemic effect on euglycemic and hyperglycemic rats and measurement of α-glucosidase level in diabetic rats.Methods ①The SD rats and alloxan-induced diabetic rats were divided into 6 groups, namely normal control group with normal saline only, negative control group with sucrose and glucose, positive control group with sucrose and glucose and Voglibose (0.043 mg·ml -1·100 g BW -1), low-dose group with sucrose and glucose and glucomannan (0.05 g·ml -1·100 g BW -1), median-dose group with sucrose and glucose and glucomannan (0.1 g·ml -1·100 g BW -1), high-dose group with sucrose and glucose and glucomannan ( 0.5 g·ml -1·100 g BW -1). Results ① Effect on sucrose tolerance test in normal rats, also expressed as ACU: All the rats with different doses of glucomannan had higher blood glucose level than that of the positive control group (P<0.05), which were 3.3, 3, and 2.7 folds higher respectively, but still lower than that in the negative control group (P<0.05) but no significant differences among low,modian and high-dose groups(P>0.05). ② Effect on glucose tolerance test in normal rats: After glucose loading, the blood glucose level elevated greatly in the negative control group, positive control group, as well as in all rats with different doses of glucomannan (P<0.05). The blood glucose levels expressed as AUC were of no statistical significances among the positive control group, low-dose group, median-dose group, high-dose group, and the negative control group. ③ Antihyperglycemic effect of glucomannan in alloxan induced diabetic rats: The amplitude of glucose reduction in the positive control group was 2.8 folds as that in negative control group, with statistical significance. The same occurred in both median-dose group and high-dose group, with 2.4 and 2.6 folds reduction as in the negative control group respectively(P>0.05). ④ Effect of glucomannan on sucrose tolerance test in alloxan-induced diabetic ra

关 键 词：葡甘聚糖 四氧嘧啶 糖尿病 动物模型 大鼠 

分 类 号：R587.1[医药卫生—内分泌]