圆瓣姜花种子胚的组织培养与快速繁殖  

Tissue culture and rapid propagation of zygotic embryo of Hedychium forrestii

作　　者：熊友华[1] 马国华[1] 刘念[2] 黄邦海[3] 

机构地区：[1]中国科学院华南植物园,广东广州510650 [2]仲恺农业技术学院,广东广州510225 [3]广州市农业技术推广中心,广东广州510520

出　　处：《广西植物》2005年第3期241-244,i008,共5页Guihaia

基　　金：广东省科技项目(20037C201029)

摘　　要：采用广西百色地区野生圆瓣姜花的种子胚作外植体,成功地进行了离体组织培养与快速繁殖,建立了高频率的丛芽繁殖体系。实验结果表明:诱导种子胚萌芽的最适培养基为MS附加1.0mg·L16BA和0.2mg·L1NAA;在MS+6BA4.0mg·L1+NAA0.2mg·L1的培养基上最有利于诱导丛芽及增殖,芽的增殖系数达到5.28;1/2MS+IBA0.5mg·L1培养基最易诱导生根,生根率达97.7%。Tissue culture of Hedychium forrestii was studied,using the zygotic embryo as explants. The results were as follows:The suitable condition for germination of zygotic embryo was in MS supplemented with 1.0 mg·L -1 6-BA and 0.2 mg·L -1 NAA;The best medium for propagation of multiple-shoot was MS+6-BA 4.0 mg·L -1+NAA 0.2 mg·L -1;1/2MS+0.5 mg·L -1 IBA could effectively promote the root emergence of the shoots,and the percentage of the root emergence accounted for 97.7%;The medium for transplanting was the mixture of perlite and peat(1∶1).

关 键 词：圆瓣姜花 种子胚 组织培养 速繁殖 

分 类 号：Q943.1[生物学—植物学]