系统性红斑狼疮患者血浆血小板活化因子水平及其与血浆脂质过氧化物水平关系的研究  

Plasma level of platelet-activating factor and its relation to plasma level of LPO in patients with systemic lupus erythematosus

作　　者：徐祖森[1] 蔡华华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院皮肤科,武汉430030

出　　处：《中国麻风皮肤病杂志》2005年第6期429-431,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的探讨系统性红斑狼疮(SLE)患者血浆血小板活化因子(PAF)水平与SLE疾病活动性及血浆脂质过氧化物(LPO)水平的关系。方法采用双抗体夹心ELISA法检测血浆PAF水平,采用硫化巴比妥酸(TBA)比色法检测血浆丙二醛(MDA)含量,并以MDA含量代表血浆LPO水平。结果SLE组血浆PAF水平(270.69±42.68)ngmL明显高于正常对照组(200.85±32.61)ngmL(P<0.05),活动期组(288.90±34.17)ngmL高于非活动期组(225.17±23.73)ngmL(P<0.05);有肾损害组血浆PAF水平(300.13±27.91)ngmL高于无肾损害组(238.30±31.01)ngmL(P<0.05)。血浆PAF水平与血浆LPO水平呈正相关(r=0.700,P<0.01),与疾病活动性指数呈正相关(r=0.730,P<0.01),与血沉(ESR)亦呈正相关(r=0.758,P<0.01)。结论SLE患者血浆PAF水平升高,PAF与血浆LPO水平、SLE活动性相关,也与SLE肾损害有关,PAF可能参与SLE的发病机制。Objective: To determine the plasma level of platelet-activating factor (PAF) in patients with systemic lupus erythematosus (SLE) and to explore its relation to plasma lipid peroxide (LPO) level and SLE activity. Methods: Plasma level of PAF was measured by sandwich ELISA and plasma LPO level was substituted by plasma malonyldialdehyde (MDA) level measured by thiobarbituric acid (TBA). Results: Plasma level of PAF was significantly increased in SLE patients (270.69±42.68)ng/mL as compared with normal controls(200.85±32.61)ng/mL(P<0.05), and was higher in active SLE patients(288.90±34.17)ng/mL than that in inactive SLE patients (225.17±23.73)ng/mL(P<0.05). Plasma level of PAF was also higher in renal involvement cases (300.13±27.91)ng/mL than that without renal disorders (238.30±31.01)ng/mL(P<0.05). Plasma level of PAF showed positive correlation with plasma LPO level (r=0.700, P<0.01), disease activity index (r=0.730, P<0.01) and ESR (r=~0.758 , P<0.01). ~Conclusion : PAF is associated with disease activity, plasma LPO level and the disorders of renal involvement. PAF might be involved in the pathogenesis of SLE.

关 键 词：血浆脂质过氧化物 系统性红斑狼疮 患者血浆 血小板活化因子(PAF) ELISA法检测 丙二醛(MDA) SLE 疾病活动性 双抗体夹心 MDA含量 正常对照组 LPO水平 活动性指数 肾损害 正相关 巴比妥酸 发病机制 活动期 比色法 mL 

分 类 号：R593.241[医药卫生—内科学]