抗病毒治疗对慢性乙型肝炎患者Ⅱ型树突状细胞及淋巴细胞亚群的影响  

Characteristics of circulating type 2 pre-dendritic cells(pDC2)and lymphocyte subsets in chronic hepatitis B patients accepting antiviral therapy

作　　者：段学章 王福生 王敏 庄辉 胡瑾华 李捍卫 

机构地区：[1]解放军第三O二医院全军艾滋病与病毒性肝炎防治重点实验室,北京100039

出　　处：《中华传染病杂志》2005年第2期125-129,共5页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金(30271230)

摘　　要：目的分析慢性乙型肝炎(慢性乙肝)患者抗病毒治疗前后外周血Ⅱ型树突状细胞(pDC2)的数量和功能变化以及淋巴细胞亚群的数量变化。方法采用流式细胞分析技术对33例慢性乙肝患者进行抗病毒治疗前后的动态观察,拉米夫定治疗组14例,干扰素α治疗组19例。检测患者外周血pDC2的比例、数量和淋巴细胞亚群数量;用体外灭活的1型单纯疱疹病毒(HSV1)刺激外周血单个核细胞(PBMCs)并与PBMCs进行24h共培养,检测培养上清液中干扰素α的产量,以评估pDC2产生干扰素α的能力;统计分析pDC2数量、功能变化及其临床意义。结果拉米夫定组和干扰素α组患者外周血pDC2的比例、数量和产生干扰素α的功能在治疗前均低于对照组(P<0.05)。抗病毒治疗后,拉米夫定组pDC2的比例和数量有提高,伴随自然杀伤(NK)细胞及CD8+T细胞数量的提高;干扰素α组pDC2的比例无明显变化,但pDC2细胞数有提高,同时伴有CD4+T细胞、NK细胞及CD8+T细胞数量的上升。结论拉米夫定和干扰素α作用机制不同,但均可提高慢性乙肝患者的pDC2、NK细胞及CD8+细胞数量。Objective To identify impacts of antiviral therapy on the frequency and interferon (IFN)-α-producing ability of circulating type 2 pre-dendritic cells(pDC2)as well as lymphocyte subsets in patients with chronic hepatitis B virus (HBV)infection. Methods Thirty-three patients with chronic HBV infection and 25 healthy individuals as controls were enrolled in our study. Two kinds of drugs, lamivudine and IFN-α, were used in antiviral regimen respectively. Absolute numbers of circulating pDC2 and lymphocyte subsets were analyzed by flow cytometry consecutively. The IFN-α-producing function of peripheral blood mononuclear cells (PBMCs) representing the circulating pDC2 was determined by ELISA assay. Results In patients with chronic HBV infection, the frequency and function of pDC2 decreased compared with those found in healthy controls. After antiviral treatment for 6 months, lamivudine seemed to induce the increase of frequency and absolute number of pDC2. The absolute numbers of peripheral CD8^+ T-cells and NK cells were simultaneously found to ascend. A similar result was also observed in chronically HBV-infected humans with 6 months duration of IFN-α therapy. Conclusions Our results showed that lamivudine or IFN-α antiviral treatment could increase the number of peripheral pDC2 and CD8^+ T-cells in the patients with chronic HBV infection.

关 键 词：Ⅱ型树突状细胞 淋巴细胞亚群 慢性乙型肝炎患者 抗病毒治疗 流式细胞分析技术 外周血单个核细胞 1型单纯疱疹病毒 慢性乙肝患者 pDC2 CD4+T细胞 干扰素α 拉米夫定 细胞数量 功能变化 治疗前后 NK细胞 PBMCs 培养上清液 

分 类 号：R512.62[医药卫生—内科学]