盐酸多柔比星脂质体注射液中磷脂膜成分的HPLC-ELSD测定  

Determination of Composition of Phospholipid Bilayer in Doxorubicin Hydrochloride Liposomes for Injection by HPLC-ELSD

作　　者：耿文飞[1] 牛长群[1] 张涛[1] 孙莉[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《中国医药工业杂志》2005年第6期359-361,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了HPLC-ELSD法测定盐酸多柔比星脂质体注射液磷脂膜中胆固醇、磷脂酰胆碱和溶血磷脂酰胆碱的含量,采用氨基色谱柱,流动相为2%草酸-乙醇-甲醇-氯仿(16∶84∶97∶3),流速1ml/min。三者的平均回收率分别为98.8%、98.7%、98.6%,RSD为1.3%、0.9%、1.4%。A HPLC-ELSD method was established for determination of cholesterin, phosphatidylcholine and lysophosphatidyl choline in phospholipid bilayer of doxorubicin hydrochloride liposomes for injection. A NH2 column was used with the mobile phase of 2% oxalic acid-ethanol-methanol-chloroform (16∶84∶97∶3) at the flow rate of 1ml/min. The average recoveries were 98.8%、98.7%、98.6%, with RSD of 1.3%, 0.9% and 1.4%, respectively.

关 键 词：盐酸多柔比星 脂质体 注射液 胆固醇 磷脂酰胆碱 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]