肺气虚证模型大鼠支气管灌洗液TNF-α和LTB_4测定的意义  

Detection of TNF-α, LTB_4 in Bronchoalveolar Lavage Fluid in Rat with Qi Deficiency of Lung

作　　者：李泽庚[1] 张杰根[1] 彭波[1] 张念志[1] 韩明向[1] 

机构地区：[1]安徽中医学院第一附属医院,合肥230031

出　　处：《天津中医药》2005年第3期226-228,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：安徽省自然科学基金资助项目(NO:010431006)

摘　　要：犤目的犦测定肺气虚证模型大鼠支气管灌洗液肿瘤坏死因子-α(TNF-α)、白三烯(LTB4)的水平,探讨TNF-α、LTB4在肺气虚证发病中的意义。犤方法犦采用慢性阻塞性肺疾病(COPD)模型大鼠的造模方法建立肺气虚证大鼠模型,经鼻腔注入肺炎克雷伯杆菌反复感染造模,实验分为对照组和模型组。采用酶联吸附试验(ELISA)方法,测定两组大鼠支气管灌洗液TNF-α、LTB4水平。犤结果犦模型组支气管灌洗液TNF-α、LTB4水平较正常组显著升高。犤结论犦TNF-α、LTB4可能参与了肺气虚证慢性气道炎症的改变,在肺气虚的发病机制中可能有重要意义。[Objective] To detect the levels of TNFα and LTB4 in bronchoalveol ar lavage fluid of rat with Qi deficiency of lung,and to explore the probable ro le in The pathogenesis of TNF-α and LTB4. [Methods] Rat with Qi deficiency of lung was induced by repeat intranasal infecting of klebsiella pneumoniae twice a week. Levels of TNF-α and LTB4 in bronchoalveolar lavage fluid of two groups were measured by ELISA . [Results] Levels of TNF-α, LTB4 in the model group w ere significantly higher than that in normal group. [Conclusion] TNF-α,LTB4 m ay involve in the chronic inflammation changes in respiratory tract in qi defici ency of lung and may contribute to the pathogenesis of qi deficiency of lung.

关 键 词：肺气虚证 肿瘤坏死因子-α(TNF-α) 白三烯(LTB4) 

分 类 号：R256.1[医药卫生—中医内科学]