活血化瘀注射液(HHI-I)对TNF-α诱发大鼠肺微血管内皮细胞黏附分子mRNA表达的影响  

Effect of HHI-I on TNF-alpha Mediated Expression of Adhesion Molecules mRNA of Pulmonary Microvascular Endothelium in Rats

作　　者：张艳军[1] 赵连根[2] 吴咸中[2] 

机构地区：[1]天津中医学院,天津300193 [2]天津市中西医结合急腹症研究所,天津300100

出　　处：《天津中医药》2005年第3期240-242,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市卫生局科研基金资助课题(98ky-9)

摘　　要：犤目的犦从调节白细胞内皮细胞过度黏附角度研究活血化瘀中药的作用机制。犤方法犦体外培养肺微血管内皮细胞,RT-PCR方法观察活血化瘀注射液对肿瘤坏死因子-α(TNF-α)诱发的体外培养大鼠肺微血管内皮细胞E-选择素、P-选择素、细胞间黏附分子ICAM-1mRNA表达的影响。犤结果犦肺微血管内皮细胞与含TNF-α培养基共同孵育后,可显著增加内皮细胞E-选择素、P-选择素、ICAM-1mRNA表达,在培养基中加入活血化瘀注射液含药血清后,则内皮细胞E-选择素、P-选择素、ICAM-1mRNA过度表达被明显抑制。犤结论犦抑制内皮细胞E-选择素、P-选择素、ICAM-1mRNA过度表达是活血化瘀重要的作用机制之一。[Objective] To elucidate the mechanism underlying the effect of Huox ue Huayu(Activating blood circulation and remove stasis) herbal medicine on th e recruitment of leukocytes in blood circulation. [Methods] The effect of HHI -I(Huoxue Huayu Injection No.I) on the expression of adhesion molecules mRNA i nduced by TNF-alpha was investigated in vitro with RT-PCR technique. [Result] In the cultured lung microvascular endothelial cells stimulated with TNF-alpha, the expression of adhesion molecules, such as E-selectin, P-selectin, ICAM-1 mRN A, was increased significantly. In contrast, the cells exposed to HHI-I simultan eously exhibited a marked lower expression of the TNF-alpha induced endothelial adhesion molecules. [Conclusion] HHI-I can inhibit the excessive expression of endothelial cell adhesion molecules.

关 键 词：活血化瘀注射液 肺微血管内皮细胞 细胞因子 黏附分子 

分 类 号：R289[医药卫生—方剂学]