盐酸埃他卡林对自发性高血压大鼠肾组织K_(ATP)通道基因表达的影响  

Effects of iptakalim hydrohloride on K_(ATP) genes expression in renal tissues of spontaneously hypertensive rats

作　　者：薛浩[1] 高敏[1] 刘国树[2] 汪海[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]解放军总医院南楼心内科,北京100853

出　　处：《中国临床药理学与治疗学》2005年第5期489-493,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家863计划重大专项(№2002AA2Z3137)

摘　　要：目的:从分子生物学水平探讨盐酸埃他卡林(iptakalimhydrohloride,Ipt)对自发性高血压大鼠(spontaneouslyhypertensiverats,SHR)肾组织KATP亚型表达的影响。方法:SHR于第12周龄进入实验,实验设Ipt1、3和9mg·kg-1·d-13个剂量组,盐酸苯那普利(benazepril)3mg·kg-1·d-1治疗组及SHR空白对照组,另设同周龄同种属正常血压大鼠(wistarKyotorat,WKY)为正常对照组,灌胃给药每天1次,连续12周,观察盐酸埃他卡林对血压和肾组织KATP亚型表达的影响。结果:SHR肾组织SUR2、Kir6.1、Kir1.1mRNA表达较WKY大鼠明显升高,盐酸埃他卡林1、3、9mg·kg-1·d-13个剂量组治疗后均可明显降低血压同时下调肾脏高表达的Kir6.1、Kir1.1mRNA水平,而对SUR2表达无明显影响。结论:盐酸埃他卡林对SHR降压治疗对肾脏的保护作用可能与其影响Kir6.1、Kir1.1基因表达有关。AIM: To investigate the effects of iptakalim hydrohloride on K_(ATP) mRNA expression in renal tissues of spontaneously hypertensive rats. METHODS: SHRs at the age of 12-week-old were treated with Ipt 1, 3, and 9 (mg·kg^(-1)·d^(-1)), benazepril 3 (mg·kg^(-1)·d^(-1)) once a day for 12 weeks, respectively. The same aged WKY rats were used as normal control. The effects of Ipt on BP and renal K_(ATP) mRNA expression were detected by RT-PCR. RESULTS: mRNA expression level of SUR2?Kir6.1 and Kir1.1 increased in SHR. After administration of 1, 3, and 9 (mg·kg^(-1)·d^(-1)) Ipt,the levels of BP were decreased,and the mRNA expression of Kir6.1 and Kir1.1 were decreased. But there was no change in mRNA expression of SUR2. In addition, there was no significantly difference of mRNA expression of Kir6.2 among the SHR groups and the WKY group. CONCLUSION: The renal protective effects of Ipt may be related to regulation of genes expression of Kir6.1 and Kir1.1.

关 键 词：盐酸埃他卡林 自发性高血压大鼠 基因表达 肾脏 ATP敏感性钾通道 

分 类 号：R966[医药卫生—药理学]