粘附分子在中性粒细胞-脐静脉内皮细胞粘附中作用机制的研究  

Roles of adhesion molecules in adhesion of polymophonuclear leukocytes-human umbilical vein endothelial cells

作　　者：张雪萍[1] 任永功[1] 刘新波[1] 石碧明[1] 曾邦雄[1] 

机构地区：[1]暨南大学医学院附属第二医院

出　　处：《中国临床药理学与治疗学》2005年第5期547-550,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：深圳市科技计划项目基金资助(№200204004)

摘　　要：目的:探讨PMN及血管内皮表面粘附分子在中性粒细胞脐静脉内皮细胞(PMNEC)粘附中的作用。方法:实验分5组:正常对照组;LPS处理组(将PMN及HUVEC用100μg·L-1LPS处理不同时间);LPS+CD11b单抗处理组;LPS+ICAM1单抗处理组;LPS+CD11b+ICAM1单抗处理组。用99mTcHMPAO标记PMNs,加入到HUVECs作用一段时间,PMNEC粘附率=溶解液放射值加入液放射值×100。结果:与对照组相比,经LPS刺激后15minPMNEC粘附性明显上升(37.45%±3.92%vs11.03%±4.15%,P<0.05),于4h处达到峰值(73.50%±6.39%)。CD11b单抗和ICAM1单抗可明显抑制这种粘附(P<0.05)。合用两种单抗有叠加效应。结论:在PMN与内皮细胞粘附的过程中,粘附分子CD11b与ICAM1起着举足轻重的作用,用单抗封闭粘附分子的作用可明显削弱细胞间的粘附。AIM: To investigate the interaction of CD11b and its ligand ICAM-1 on PMN-HUVEC adhesion and their action mechanism. METHODS: Human neutrophils were isolated from fresh heparinized blood of healthy volunteers by dextran sedimentation followed by Ficoll-Hypaque gradient centrifugation. The polymophonuclear leukocytes- human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical veins by (0.25)% trypsin and then cultured in RPMI1640. There were five groups in this experiment for the adhesion assay: control group, LPS treated group (PMNs and HUVECs were stimulated with 100 (μg·L^(-1)) LPS in different time), CD11bMoAb pretreated group, ICAM-1 MoAb pretreated group, combined CD11b and ICAM-1 MoAb pretreated?group. The?rate?of?PMN-HUVEC?adhesion was calculated as formula after radiolabelling PMNs with^(99m) Tc-HM-PAO. adherence(%)=cpm lysate/cpm added×100. RESULTS: PMN-HUVEC adhesiveness increased after stimulated for 15 min compared with that in the control group ((37.45)%±(3.92)% vs (11.03)%±(4.15)% P<(0.05)), and it reached the peak after 4 hours ((73.50)%±(6.39)%). MoAb to CD11b and ICAM-1 significantly inhibited this adhesiveness (P<(0.05)). Moreover, an additive effect was observed when two MoAbs were used together (P<(0.05)). CONCLUSION: CD11b and ICAM-1 play a crucial role in the course of PMN-HUVEC adhesion, and the adhesiveness between cells is significantly attenuated after MoAb blocked the action of adhesion molecules.

关 键 词：中性粒细胞 脐静脉内皮细胞 CD11B 细胞间粘附分子-1 粘附 脂多糖 单克隆抗体 

分 类 号：R966[医药卫生—药理学]