冠心宁片对气滞血瘀大鼠抗血小板聚集和保护血管内皮作用  

Inhibitive and protective effects of guanxinning tablets on platelet aggregation and vascular endothelium in qi stagnation and blood stasis rats

作　　者：陈民利[1] 寿旗扬[1] 潘永明[1] 张建兵 桑睿 管敏文 吴碧元 

机构地区：[1]浙江中医学院动物实验研究中心 [2]正大青春宝药业有限公司

出　　处：《中国临床药理学与治疗学》2005年第5期586-589,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省医药卫生专项基金项目(№2003ZC040)

摘　　要：目的:观察冠心宁片对气滞血瘀模型大鼠血小板聚集、血液凝固和血管内皮分泌功能的影响。方法:大鼠连续15d给予低(0.3g·kg-1)、中(0.6g·kg-1)、高(1.2g·kg-1)剂量的冠心宁片后,用皮下注射肾上腺素加冰水浸泡致大鼠气滞血瘀,测定血小板聚集率、粘附率、PT、TT、APTT和血浆TXB2、6KetoPGF1α、NO、ET以及血清SOD活性、MDA含量。结果:冠心宁片能使气滞血瘀大鼠血小板聚集率和粘附率明显下降,PT、TT、APTT明显缩短,血清中SOD活性明显提高、MDA含量明显下降,血浆NO浓度明显提高、ET水平明显降低,TXB2的含量上升、TXB2与6KetoPGF1α的比值下降。结论:冠心宁片可通过降低气滞血瘀大鼠血小板聚集和血液凝固性增强自由基的清除能力,改善血管内皮细胞的分泌功能,起到保护血管内皮的作用。AIM: To observe the effects of the guanxinning tablets on platelet aggregation, blood coagulation and vascular endothelial secretion function in qi stagnation and blood stasis rats. METHODS: Rats were treated with high ((1.2 g·kg^(-1))), middle ((0.6 g·kg^(-1))) and low ((0.3 g·kg^(-1))) doses of guanxinning for fifteen days, and then qi stagnation and blood stasis rat model was established by adrenalin subcutaneous injection and ice water immersion. Spontaneous platelet aggregation and adhesion rate, prothrombin time (PT), thromboplastin time (TT), activated partial thromboplastin time (APTT), level of plasma thromboxane B_2 (TXB_2), 6-Keto-PGF_(1α), nitric oxide (NO), endothelin (ET), content of MDA and activity of serum superoxide dismutase (SOD) were determined. RESULTS: Spontaneous platelet aggregation and adhesion rate in guanxinning rats was remarkably descended. PT, TT, APTT content of NO and TXB_2 in plasma were elevated significantly, the content of MDA in serum was increased and activity of SOD was reduced obviously. And the content of ET in plasma was decreased obviously, and the rate of (TXB_2) to 6-Keto-PGF_(1α) in plasma was digressive. CONCLUSION: Guanxinning contributes to inhibit platelet aggregation and blood coagulation in rat models, to boost elimination for free radical, and to improve secretion function of vascular ehdothelial cells.

关 键 词：冠心宁片 气滞血瘀 大鼠 血小板聚集 血管内皮 

分 类 号：R966[医药卫生—药理学]