两个新的钙感觉受体基因多态性与中国男性骨密度相关性研究  

Association study of two new polymorphisms in calcium-sensing receptor gene and bone mineral density in chinese men

作　　者：吴涤[1] 洪秀梅[1] 张秀清[1] 李志平[1] 臧桐华[1] 

机构地区：[1]安徽医科大学生物医学研究所,安徽合肥230032

出　　处：《疾病控制杂志》2005年第3期246-249,共4页Chinese Journal of Disease Control and Prevention

基　　金：安徽省自然科学基金资助项目(01043802)

摘　　要：目的研究钙感觉受体(CaSR)基因两个新位点多态性与中国男性骨密度的关系。方法采用双能X线吸收仪对374名研究对象进行腰椎及股骨扫描,应用PCRRFLP方法检测CaSR基因R990G和E1011Q两个多态位点基因型,用广义线性模型分析CaSR基因多态性与第2～4腰椎及股骨骨密度关系。结果在调整环境危险因素前后,均未发现CaSR基因的两个位点多态性与男性股骨颈、股骨柄和股骨三角区及第2～4腰椎的骨密度相关。结论在中国男性人群中CaSR基因R990G和E1011Q位点多态性与股骨及腰椎骨密度无显著性相关。Objective To investigate the association of two new sites polymorphism of calcium-sensing receptor gene and bone mineral density (BMD) in chinese men. Methods The bone mineral density of lumbar spine (L2-4) and femur bone was measured by dual-energy X-ray absorptiometry in 374 study subjects. PCR-restriction fragment length polymorphism (PCR-RFLP) was applied to examine R990G and E1011Q two sites polymorphism of the CaSR gene. Generalized linear model was used to analysis the association of this polymorphism and the bone mineral density of the lumbar spine (L2-4) and femur. Results Without or with adjustment for environmental covariates, no significant correlation was found between the two sites of the CaSR gene and BMD, including femoral neck BMD, ward triangle BMD and Femoral shaft BMD. Conclusions There are no significant relationship between R990G and E1011Q polymorphisms of CaSR gene and femoral BMD and lumbar spine BMD in Chinese men population.

关 键 词：中国男性 受体基因多态性 相关性研究 PCR-RFLP方法 位点多态性 双能X线吸收仪 环境危险因素 钙 腰椎骨密度 研究对象 多态位点 密度关系 模型分析 男性人群 骨扫描 基因型 股骨颈 未发现 三角区 股骨柄 显著性 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学] R681.02[医药卫生—基础医学]