小鼠I-A^kII类分子限制性MAGE-3T表位筛选  

Screening and identification of murine MAGE-3 derived,MHC class II restricted peptide epitope

作　　者：李强[1] 张轶[1] 张奕[1] 陈雪华[1] 李建芳[1] 顾琴龙[1] 朱正纲[1] 刘炳亚[1] 

机构地区：[1]上海第二医科大学附属瑞金医院外科上海消化外科研究所,上海200025

出　　处：《现代免疫学》2005年第1期19-23,共5页Current Immunology

基　　金：国家自然科学基金项目(30170915)

摘　　要：为了筛选小鼠MAGE-3抗原来源的MHCII类分子限制性多肽表位。用计算机模拟设计,从MAGE-3中选取得分最高的5个候选表位。根据诱导T淋巴细胞增殖能力、对肿瘤细胞的特异性识别及释放细胞因子能力评价多肽的免疫原性及免疫反应性。结果DC负载MAGE-322-36能够强效诱导T淋巴细胞增殖,DC负载MAGE-322-36诱导的T细胞以MHCII类分子限制性方式、特异性识别表达MAGE-3抗原的肿瘤细胞。DC负载MAGE-322-36诱导的T细胞群主要为CD4+Th1细胞,同时天然免疫系统中的NK、NKT、γ/δT细胞也得到活化。从小鼠MAGE-3抗原中筛选出MHCII类分子限制性多肽表位MAGE-322-36。To screen murine MAGE,3 derived, MHC class II restricted peptide epitope, we used a MHC class II epitope prediction algorithm to select five candicate peptides according to the score. The immunogenicity and immune reactivity of peptide were evaluated on the basis of T,cell proliferation assay, specific recognition of tumor cells and cytokines release. The experimental results showed that DC loaded with MAGE,322,36 elicited potent T cells proliferation, and MAGE,322,36 pulsed DC activated T cells specificially recognized MAGE,3 expressing tumor cells in MHC class II restricted manner. MAGE,322,36 pulsed DC induced T cells population was mainly CD4+ Th1 cells, and NK, NKT, γ/δT in the innate immune system were activated concomitantly. It concludes that MAGE,322,36 was identified as a MHC class II restricted epitope.

关 键 词：MAGE-3 TH表位 筛选 

分 类 号：R392.11[医药卫生—免疫学] R730.3[医药卫生—基础医学]