刺葡萄愈伤组织的分化、芽的增殖及生根培养  

Callus differentiation,bud multiplication and rooting culture of Vitis davidii Fox

作　　者：刘金亮 吕长平[2] 石雪晖[2] 徐艳[2] 陈冲[2] 

机构地区：[1]岳麓山风景名胜管理处,湖南长沙410012 [2]湖南农业大学园艺园林学院,长沙410128

出　　处：《作物研究》2005年第2期106-108,共3页Crop Research

基　　金：湖南省教育厅资助项目;编号99B028。

摘　　要：以刺葡萄愈伤组织为材料,在B5培养基上附加不同的激素种类及浓度配比,研究了刺葡萄芽的诱导、增殖和生根培养。结果表明,刺葡萄愈伤组织再生苗的分化率很低,仅在B5+0.5mg/LZT+0.1mg/LNAA上有少量分化,分化率为13.3%;增殖培养以B5+BA1.0mg/L+NAA0.05mg/L为佳,4周可增殖6.4倍;生根培养以B5+0.5mg/LIBA+0.02mg/LNAA为好,4周平均生根3.4条,根粗达2.4mm。<Abstrcat> Calli of Vitis davidii Fox were used as the material and different plant growth regulators were added at various concentrations into B_5 medium to study bud induction,multiplication and rooting culture.The results showed that calli differentiated in a very frequency and only in the medium supplemented with 0.05 mg/L ZT and 0.1 mg/L NAA in which the frequency of differentiation was 13.3%.The optimum medium for multiplication was B_5 supplemented with 1.0 mg/L BA and 0.05 mg/L NAA, in which the plantlets could propagate 6.4 times in 4 weeks.The most suitable medium for rooting was B_5 supplemented with 0.5 mg/L IBA and 0.02 mg/L NAA in which the plantlet could produce 3.4 roots on average with the thickness of the root up to 2.4 mm.

关 键 词：刺葡萄 愈伤组织 分化 增殖 生根培养 培养基 培养条件 

分 类 号：S663.1[农业科学—果树学]