锌对缺血再灌注损伤家兔心肌金属硫蛋白-1基因表达的影响  

Effect of zinc on the gene expression of myocardial metallothionein-1 after ischemia reperfusion injury in rabbits.

作　　者：沈炳玲[1] 颜利求[1] 刘继英[2] 邢冬红[2] 秦毅[2] 

机构地区：[1]天津医科大学病理生理教研室,天津300070 [2]天津医科大学医学检验系,天津300204

出　　处：《中国心血管杂志》2005年第3期161-163,共3页Chinese Journal of Cardiovascular Medicine

基　　金：天津医科大学科研基金资助(编号:2003KY27)

摘　　要：目的研究锌对家兔心肌缺血再灌注损伤的保护作用。方法采用RT-PCR技术,检测分析金属硫蛋白-1(MT-1)基因在家兔缺血再灌注损伤心肌的表达及锌对其基因表达的调节。结果各组家兔心肌组织中均有MT-1基因的表达,缺血再灌注组MT-1基因的表达较正常对照组明显下降(P<0.01),补锌再灌注组MT-1基因的表达较缺血再灌注组明显上调(P<0.01)。结论锌对家兔心肌缺血再灌注损伤的分子机制中,调节其心肌组织中MT-1基因转录水平的表达可能是一条重要途径。Objictive To explore the molecular mechanism of protection of zinc against myocardial ischemia reperfusion injury (MIRI). Methods the myocardial metallothionein-1(MT) gene and regulation by zinc were determined with reverse transcription-polymerase chain reaction(RT-PCR) in MIRI rabbits. Results The myocardial MT-1 mRNA was expressed in all groups. the myocardial MT-1 mRNA in MIRI group was significantly lower than contro(P<0.01). After zinc supplementation, the content of the myocardial MT-1 mRNA was increased significantly(P<0.01). Conclusion The results suggest that the regulation of myocardial MT-1 gene by zinc may be way contributed to the mechanism of protection by zinc in MIRI.

关 键 词：锌 缺血再灌注损伤 金属硫蛋白-1 反转录聚合酶链式反应 

分 类 号：R541[医药卫生—心血管疾病]