电针“内关"对急性心肌缺血大鼠心肌c-fos基因表达的影响  

Effect of electroacupuncture at "Neiguan" (PC 6) on expression of c-fos gene in myocardium of the rat with acute myocardial ischemia

作　　者：谢芳[1] 梁勋厂[1] 吴红金[2] 茹立强[1] 

机构地区：[1]华中科技大学同济医学院,武汉430030 [2]北京中医药大学附属中西医结合医院

出　　处：《中国针灸》2005年第5期355-358,共4页Chinese Acupuncture & Moxibustion

基　　金：国家中医药管理局中医药科学技术研究基金资助项目:2000-J-B-01

摘　　要：目的:探讨电针“内关”改善急性心肌缺血的机制。方法:将96只大鼠随机分为假手术组、心肌缺血模型组、心肌缺血模型加电针组,采用结扎冠状动脉左前降支建立急性心肌缺血实验模型,造模成功后,电针双侧“内关”。用生化方法检测血清心肌酶活性,逆转录聚合酶链反应(RT-PCR)方法检测心肌中c-fos基因的表达。结果:心肌缺血模型组血清心肌酶活性和心肌c-fosmR—NA表达显著高于假手术组(P<0.05),电针后降低,差异有显著性意义(P<0.05)。结论:电针“内关”改善急性心肌缺血的机制与下调心肌组织c—fosmRNA的表达有关。Objective To explore the mechanism of electroacupuncture at 'Neiguan' (PC 6) improving acute my-ocardial ischemia. Methods The rats were randomly divided into a sham operation group , a myocardial ischemia model group and a myocardial ischemia model plus electroacupuncture group. The acute myocardial ischemia model was developed by ligation of the descending anterior branch of the coronary artery, and electroacupuncture was given at bilateral 'Neiguan' (PC 6). Serum myocardial enzymes was determined by biochemical method and the expression of c-fos mRNA in myocardium was detected by using reverse transcription polymerase chain reaction (RT-PCR). Results The activities of serum myocardial enzymes and the expression of c-fos mRNA in ischemic myocardium were significantly increased as compared with those in the sham operation group (P<0. 05), and after electroacupuncture they were significantly decreased ( P < 0. 05). Conclusion The mechanism of electroacupuncture at 'Neiguan' (PC 6) improving acute myochadial ischemia is possibly related with down-regulation of expression of c-fos mRNA in myocardium.

关 键 词：电针 穴 内关 心肌缺血/针灸疗法 原癌基因蛋白质c-fos类/代谢 原癌基因蛋白质c-fos类/针灸效应 基因表达 

分 类 号：R245.97[医药卫生—针灸推拿学]