1.8GHz微波对四种化学诱变剂致DNA的损伤作用  

Influence of 1.8 GHz microwave on DNA damage induced by 4 chemical mutagens

作　　者：王保红[1] 鲁德强[1] 金力奋[1] 郑伟[1] 楼建林[1] 邓红平[1] 李晴宇[2] 何继亮[1] 

机构地区：[1]浙江大学医学院劳动卫生与环境卫生研究所,杭州310006 [2]杭州市第一人民医院药剂科

出　　处：《中华劳动卫生职业病杂志》2005年第3期163-166,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：浙江省自然科学基金资助项目(300434)

摘　　要：目的观察1.8GHz[比吸收率(SAR)为3Wkg]微波(MW)对4种化学诱变剂[丝裂霉素C(MMC)、博莱霉素(BLM)、4硝基喹啉氧化物(4NQO)、甲基甲烷磺酸酯(MMS)]诱发的人外周血淋巴细胞DNA损伤的影响。方法采用彗星试验体外实验分别在暴露后0h和21h检测1.8GHz微波、4种化学诱变剂及微波联合4种诱变剂所诱发的人淋巴细胞DNA损伤。所用的指标为尾长(TL)和尾相(TM)。微波辐射和化学诱变剂暴露时间分别为2h和3h。结果微波组所诱发的DNA损伤与对照组相比,差异无统计学意义(P>0.05);微波分别与MMC和4NQO的联合暴露组所诱发的DNA损伤明显高于相应浓度的MMC组和4NQO组,差异有统计学意义(P<0.01或P<0.05);但微波对BLM和MMS所诱发DNA损伤的增强效应不明显,差异无统计学意义(P>0.05)。结论1.8GHz(SAR为3Wkg)微波暴露2h并不诱发人淋巴细胞DNA损伤,但能增强MMC、4NQO的DNA损伤效应。Objective To observe the influence of 1.8 GHz microwave(MW) specific absorption rate (SAR,3 W/kg) on human lymphocytes DNA damage induced by 4 chemical mutagens[mitomycin C(MMC),bleomycin(BLM),methyl methanesulfonate(MMS),and 4-nitroquinoline 1-oxide(4NQO)]. Methods Comet assay in vitro was used to detect human lymphocyte DNA damage induced by 1.8 GHz MW,4 chemical mutagens,and MW plus 4 chemicals 0 h and 21 h respectively after exposure.The time exposed to MW or mutagens was 2 h or 3 h respectively.The results were showed by tail length(TL) and tail moment(TM). Results The difference of DNA damage between MW group and control group was not statistically significant(P>0.05).DNA damages in MW plus MMC groups and MW plus 4NQO groups were significantly greater than those in the corresponding concentrations of MMC groups and 4NQO groups(P<0.01 or P<0.05).However,MW did not enhance DNA damage induced by MMS and BLM(P>0.05). Conclusion Exposure to 1.8 GHz(SAR,3 W/kg) microwave may not induce human lymphocyte DNA damage,but could enhance DNA damage induced by MMC and 4NQO.

关 键 词：化学诱变剂 人淋巴细胞DNA损伤 损伤作用 甲基甲烷磺酸酯 诱发DNA损伤 DNA损伤效应 4NQO 统计学 丝裂霉素 比吸收率 博莱霉素 硝基喹啉 人外周血 体外实验 彗星试验 暴露时间 微波辐射 增强效应 MMC 氧化物 暴露后 对照组 

分 类 号：R594.8[医药卫生—内科学]