联检C100－3抗体、第2代抗体与HCV RNA诊断丙型肝炎的比较  

COMPAPISONS BETWEEN POLYMERSAE CHAIN REACTION FOR HCV RNA AND RADIOIMMUNOASSAYS FOR C100-3 AB OR SECOND GENERATION AB IN DIAGNOSIS OF HEPATITIS C

作　　者：孟宪镛[1] 姚登福[1] 倪润洲[1] 葛政举[1] 伊东进 清水一郎[1] 

机构地区：[1]江苏省南通医学院附属医院,日本德岛大学医学部

出　　处：《中华传染病杂志》1994年第2期82-85,共4页Chinese Journal of Infectious Diseases

摘　　要：对江苏省南通市不同人群７７０例份血清联合检测Ｃ１００－３抗体和第２代抗体，并对其中抗－ＨＣＶ阳性和非甲非乙型肝炎患者用多聚酶链反应（ＲＣＲ）法检测了ＨＣＶＲＮＡ。在８９例急性肝炎中。检出的１４例急性丙型肝炎（丙肝）ＨＣＶＲＮＡ全数阳性，第２代抗体２例阳性，Ｃ１００－３抗体均阴性。慢性肝炎及肝炎后肝硬化２３０例共检出ＨＣＶ感染２４例（１０．４％），４５１例献血员检出丙肝３例（０．７％），以上两组患者３项标记阳性率相近。在检出的４２例ＨＣＶ感染中，仅１４例有输血史。比较３项标记，ＰＣＲ法检测ＨＣＶＲＮＡ最灵敏，对急性丙肝尤有诊断价值，并能直接反映ＨＣＶ复制；抗－ＨＣＶ可用于慢性ＨＣＶ感染的检测，第２代抗体优于Ｃ１００－３抗体。The nested polymerase chain reaction(PCR) ssay for HCV RNA was employed inthe cases confirmed antibodies to HCV or with non-A non-B hepatitis after simul-taneous detections with radioimmunoassays for C100-3 antibody(c100-3)Ab)and antibody in second generation Kit in 770 samples from the subjects of different groups inNantong City。In 89 HCV RNA positive cases with acute hepatitis 14 cases with acutehepatitis C were all HCV RNA positsve。There were 24 cases(10.43%)with HCV infec-tion found in 230 cases of chronic hepatitis or post-hepatitis cirrhosis,and 3 cases withhepatitis C(0.66%)were found in 451 blood donors. Howerver,no significant differen-ce was demonstrated in the positive rates of the 3 parameters between chronic hepatitisor cirrhosis cases and the blood donors. In all the 42 detected HCV positive cases,only14 cases had had history of blood transfusions.Among the 3 parameters,PCR for HCVRNA is the most sensitive one, especially for the diagnosis of acute hepatitis C,and itcan directly confirm the replication of HCV. Radioimmunoassays for antibodies to HCVare of value in the diagnosis of chronic HCV infection, and the second generation kitis superior to C100-3 Ab.

关 键 词：丙型肝炎病毒 RNA 丙型肝炎 

分 类 号：R512.630.4[医药卫生—内科学]