慢性乙型肝炎患者血清及肝组织中乙型肝炎病毒核心基因的研究  

STUDY OF HEPATITIS B VIRUS CORE IN THE LIVER TISSUE AND SERA OF CHONIC HEPATITIS PATIENTS

作　　者：张蕾青[1] 何丽芳[1] 李雪萍[1] 胡德昌[1] 闻玉梅[1] 

机构地区：[1]上海医科大学,上海医科大学中山医院,贵州遵义医学院微生物学教研室

出　　处：《中华传染病杂志》1994年第2期65-68,共4页Chinese Journal of Infectious Diseases

基　　金：国家教委博士基金

摘　　要：３６例肝穿刺诊断为慢性乙型肝炎患者的肝组织及血清，用ＨＢＶ全基因核酸杂交法及聚合酶链反应（ＰＣＲ）法扩增了病毒的ＰｒｅＣ／Ｃ区基因片段。发现ＰＣＲ法扩增后再经Ｓｏｕｔｈｅｒｎ吸印转移及核酸杂交（ＰＣＲ－ＳＢＨ）可显著提高检出ＨＢＶ基因的敏感性。对５例无任何血清ＨＢＶ标记者证实在其肝内存在ＨＢＶ基因。用ＡｖａⅡ，ｓａｕ３ＡＩ，ＸｍｎＩ，ＢｓｔＮＩ及ＴａｑＩ酶切图谱分析上述病例的ｃ基因，与ｐＡＤＲ－１Ｃ基因比较，有４例（５份标本）有Ｓａｕ３ＡＩ的异常酶切位点，提示ＨＢＶ不仅ＰｒｅＣ区存在变异株，Ｃ基因中亦可有变异。Polymerase chain reaction(PCR)was used to amplify the pr-core and core genefrom the liver biopsy tissues and serum samples from 36 chronic hepatitis B patients。PCR amplification and subsequent Southern blotting and DNA hybridization(PCR-SBH)was significantly more sensitive than the direct DNA hybridization with HBVDNA probe. In 5 cases who had no HBV serological marker,HBV DNA was detectedin their liver tissues by PCR-SBH. Five restriction endonucleases (Ava II, Sau 3AI,XmnI,Bst NI and Taq I)were used to analyse the amplified core gene from these patients.Compared to the sequence of the prototype of adr subtype of HBV (pADR-1),there werevariations in the restriction site of Sau 3AI in 4 cases(5 samples).These data sugges-ted that not only pre-core gene variations were found in HBV,but also variations in thecore gene could be detected.

关 键 词：乙型肝炎病毒 核心基因 肝炎 

分 类 号：R512.62[医药卫生—内科学]