原位杂交法检测艾滋病尸检组织中病毒核酸  

DETECTION OF HIV-1 RNA IN TISSUE SECTIONO OF A CHINESE PATIENT WITH AIDS BY IN SITU HYBRIDIZATION

作　　者：周先志[1] 王松山[1] 王凝芳[1] 孙中和[1] 朱纯吾[1] 朱关福[1] 

机构地区：[1]北京解放军第三○二医院,解放军军事医学科学院

出　　处：《中华传染病杂志》1994年第3期127-130,共4页Chinese Journal of Infectious Diseases

摘　　要：对曾用免疫组织化学方法检测了人免疫缺陷病毒１型（ＨＩＶ－１）抗原的１例艾滋病尸检病例的脑、肝、肾、心、肺、胰、肠、脾、胸腺、淋巴结和兰尾等组织标本，采用地高辛标记的ＨＩＶ－１ｃＤＮＡ探针检测病毒ＲＮＡ。结果发现在脑组织神经胶质细胞和星形细胞，肠上皮细胞以及淋巴结、脾和胸腺的淋巴细胞ＨＩＶ－１ＲＮＡ阳性。Human immunodeficiency virus-1(HIV-1)gp120 and P17 antigens were studiedby immunohistochemistry in brain,liver,heart,lung,thymus,gut,spleen,appendix,kidney,lymph nodes and pancrease tissue from a chinese patient with acquiredmm modeficiency syndrome (AIDS). Among the tissue,HIV-1 RNA was detected by insitu hybridization with a digoxigenin-labeled HIV-1 cCNA probe. The results confirmedthe presence of HIV-1 RNA in glial cells and astrocytes of the brain,gut epithelialcells,lymphocytes from lymph nodes,spleen and thymus

关 键 词：艾滋病 核酸 原位杂交 艾滋病毒 

分 类 号：R512.91[医药卫生—内科学]