反相高效液相色谱法测定烟叶中茄尼醇的含量  

Reverse Phase HPLC Determination of Solanesol in the Extracts of Tobacco Leaves

作　　者：武永昆[1] 张征[1] 苏鹏娟 尹海川[1] 林军[1] 

机构地区：[1]云南大学应用化学系,云南昆明650091 [2]云南中科生物产业有限公司,云南昆明650106

出　　处：《云南民族大学学报（自然科学版）》2005年第3期275-277,共3页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：云南省技术创新人才基金资助项目(2001PY011);云南大学校基金资助项目(2003Q001A).

摘　　要：采用反相高效液相色谱法研究了测定烟叶提取物中茄尼醇含量的方法.条件为:Hypersil ODS 柱,流动相为甲醇∶乙醇=75∶25(v/v),流速1.0 mL/min,紫外检测波长211 nm,柱温30℃.茄尼醇的质量浓度为2.16～34.56 mg/L时,峰面积与质量浓度具有良好的线性关系,相关系数为0.99998.方法的检出限(S/N≥3)为0.09 mg/L,相对标准偏差(RSD)为 0.42%(n=6),平均回收率为 99.2%.该方法快速、简便、准确.A reversed-phase high performance liquid chromatographic method was developed for the determination of solanesol in the extracts of tobacco leaves. The chromatographic analysis was carried out using a Hypersil ODS column at 30℃.The mobile phase was methanol-alcohol (75∶25,v/v), at a flow rate of 1.0mL/min and UV detection at (211nm). The experimental results showed a good linear relationship between the peak area and mass concentration of the solanesol within the range of 2.16～(34.56mg/L) (r=0.99998).The lowest limit of detection (S/N≥3) was (0.09mg/L).The relative standard deviation (RSD) was 0.42%(n=6).The average recovery was 99.2%.The method is rapid, simple and accurate.

关 键 词：反相高效液相色谱 茄尼醇 烟叶提取物 

分 类 号：O658[理学—分析化学] R93[理学—化学]