肾病患儿外周血单个核细胞产物对大鼠肾小球上皮细胞的影响  

EFFECT OF PERIPHERAL BLOODMONONUCLEAR CELL PRODUCTS OFNEPHROTIC PATEINTS ON RAT GLOMER-ULAR EPITHELIAL CELL

作　　者：王丹[1] 杨霁云[1] 王宝琳[1] 

机构地区：[1]北京医科大学第一临床医学院妇儿医院,广州军区总医院儿科

出　　处：《中华儿科杂志》1994年第2期83-85,共3页Chinese Journal of Pediatrics

摘　　要：为了从细胞水平探索循环的免疫物质在蛋白尿发生中的作用，应用（３５）￣Ｓ和３￣Ｈ－亮氨酸双同位素标记技术，观察１５例激素敏感型肾病综合征（简称肾病）患儿的外周血单个核细胞（ＰＢＭＣ）培养上清液对大鼠肾小球上皮细胞（ＧＥＣ）合成含硫化合物的影响，结果表明：肾病极期未经激素治疗组（９例）培养上清明显促进ＧＥＣ掺入（３５）￣Ｓ（Ｐ＜０．０１），激素治疗组（６例）与正常对照组比较无显著差异。提示激素敏感型肾病患儿ＰＢＭＣ的产物可影响ＧＥＣ的生物活性。AbstractTo identify the role of circulating immunefactor(s) on the mechanism of proteinuna,thesupernatant of peripheral blood mononuclear cell(PBMC) was obtained from 15 steroid-responsive pa-tients with idiopathic nephrotic syndrome,and its ef-fect on sulfated compounds synthesis by cultured ratglomerular epithelial cell(GEC)was studied by (35)￣S,3￣H-Leucine dual-isotope lablelling technlque.Theresult showed that the supernatant of PBMC from 9pateints without steroid treatment increased(35)￣S up-take by rat GEC(P<0.01),while there was no signifi-cant differencc in(35)￣S uptake between the supernatantof PBMC from 6 patients treated with prednisone(1.5～2.0 mg / kg / d)and 6 normal controls.These re-sults indicate that the products of PBMC from pa-tients with steroid-responsive nephrotic syndromecan affect the biological fonction ofcultured rat GEC.

关 键 词：肾病综合征 单核细胞 上皮细胞 

分 类 号：R726.92[医药卫生—儿科]