家兔血液中西咪替丁的微透析-毛细管电泳测定研究  

Determination of Unbound Cimetidine in Rabbit Blood by Capillary Electrophoresis with Microdialysis

作　　者：罗教华[1] 舒为群[1] 章竹君[2] 陈丁龙[2] 

机构地区：[1]第三军医大学环境卫生学教研室,重庆400038 [2]西南师范大学分析科学研究所,重庆400715

出　　处：《分析科学学报》2005年第3期253-255,共3页Journal of Analytical Science

基　　金：国家科学技术部基金(No.2003BA310A05)

摘　　要：本文报道用微透析毛细管电泳测定家兔血液中的西咪替丁。研究发现,采用如下条件:100mmol/LpH2.5的磷酸盐缓冲液,分离电压17kV,电动进样9kV×9s,西咪替丁与雷尼替丁能达到基线分离,据此以雷尼替丁为内标建立了一种测定西咪替丁的方法。西咪替丁与雷尼替丁峰面积比与相应的西咪替丁浓度在8.0×10-9～4.0×10-7g/mL范围内呈线性关系(r=0.998),检出限为5.0×10-9g/mL。药物代谢动力学参数由“NDST21”计算软件求得,代谢符合一室开放模型。The study describes a microdialysis system coupled with capillary electrophoresis for monitoring of cimetidine in test rabbit blood. Under the conditions of 100mM phosphate buffer, pH 2.5, applied voltage 10 kV and injection voltage 9 kV×9 s, cimetidine and ranitidine can be baseline separated. The peak area ratio of cimetidine and ranitidine vs. concentration of cimetidine was linear over the range of 8.0×10 -9 ～4.0×10 -7 g/mL with a detection limit of 5.0×10 -9 g/mL. The pharmacokinetics parameters of cimetidine were calculated utilizing the pharmacokinetics software “NDST-21”. The concentration-time curve of cimetidine was in accordance with one-compartmantal open model.

关 键 词：微透析 毛细管电泳 西咪替丁 药物代谢 

分 类 号：O657.8[理学—分析化学] R975[理学—化学]