手性药物山莨菪碱的毛细管电泳拆分研究  

The Enantiomeric Separation of Anisodamine by Capillary Electrophoresis

作　　者：吴俊森[1] 林秀丽[2] 

机构地区：[1]山东建筑工程学院环工系,济南250014 [2]山东大学药学院,济南250012

出　　处：《分析科学学报》2005年第3期277-279,共3页Journal of Analytical Science

摘　　要：以新型环糊精衍生物单3O苯基胺甲酰基βCD为手性选择剂,用毛细管区带电泳法对手性药物山莨菪碱进行了拆分。考察了手性选择剂浓度、缓冲液pH值和浓度及有机溶剂对拆分的影响。结果表明,手性选择剂浓度和缓冲液pH值是影响药物对映体分离的重要因素,有机溶剂亦对分离有很大影响。单3O苯基胺甲酰基βCD对山莨菪碱的分离度为2.16。Anisodamine is separated using a new derivative of mono-3-O-phenylcarbamoyl-β-CD as chiral selector. The effect of the concentration of chiral selector, pH, the concentration of buffer and organic modifier on resolution was studied. The results show that the chiral resolution(Rs) is strongly influenced by the concentrations of mono-3-O-phenylcarbamoyl- β -CD and the pH of the background electrolyte. Under the same experimental conditions, anisodamine cannot be separated by β -CD. This shows that the chiral separating ability of mono-3-O-phenylcarbamoyl-β-CD is stronger. The recognition mechanism of mono-3-O-phenylcarbamoyl-β-CD on these chiral drugs in the cyclodextrin-modified capillary zone electrophoresis was discussed briefly.

关 键 词：毛细管区带电泳 手性分离 手性药物 手性添加剂 山莨菪碱 

分 类 号：O657.8[理学—分析化学] R917[理学—化学]