乌桕δ-羟基赖氨酸的鉴别及其含量测定  

Identification and Determination of δ-Hydroxylysine from Sapium Sebiferum (L.) Roxb

作　　者：霍光华[1] 李艳[2] 郭成志[1] 

机构地区：[1]江西农业大学分析测试中心,南昌330045 [2]江西农业大学成人教育学院,南昌330045

出　　处：《分析科学学报》2005年第3期307-309,共3页Journal of Analytical Science

基　　金：江西省教育厅资助项目

摘　　要：本文报道采用溶剂提取和色谱法分离乌桕特殊氨基酸,然后利用保留时间、已知物峰高增加和核磁共振波谱法定性,并设计氨基酸离子交换色谱短程序定量测定乌桕特殊氨基酸。结果证明,乌桕中存在的特殊氨基酸为δ羟基赖氨酸,而且是乌桕的一种非常见蛋白氨基酸。To identify and analyze quantitatively a special amino acid of Chinese tallow leaf, the amino acid was extracted using solvents, separated using chromatography, identified by retention time, peak height overlapping method and nuclear magnetic resonance spectrum. A short program of ion exchange chromatography was designed for its quantitative determination. The results showed the amino acid is δ -hydroxylysine,which is a no normal protein amino acid in Chinese tallow.

关 键 词：乌桕 δ-羟基赖氨酸 鉴别和定量 

分 类 号：O657.7[理学—分析化学] Q517[理学—化学]