心肌炎及扩张型心肌病中人类小RNA病毒检测的研究  

DETECTION OF HUMAN PICORNAVIRUSES INMYOCARDITIS AND CARDIOMYOPATHY BYPOLYMERASE CHAIN REACTION

作　　者：柯碧波 吴秉铨[1] 宫恩聪[1] 钟镐镐[1] 鄂文[1] 刘淑平[1] 衡万杰[1] 叶鸿瑁[1] 秦宝贵 韩玲[1] 

机构地区：[1]北京医科大学病理学教研室,北京武警总医院儿科,北京安贞医院小儿心脏科

出　　处：《中华儿科杂志》1994年第3期144-146,共3页Chinese Journal of Pediatrics

摘　　要：利用聚合酶链反应（ＰＣＲ）技术对５８例临床疑为病毒性心肌炎、４例诊断为扩张型心肌病患儿的血液、心肌组织及疑为病毒性心肌炎的１例胎儿羊水标本、２例尸检心肌组织蜡块分别进行人类小ＲＮＡ病毒的检测。结果显示：临床疑为病毒性心肌炎的５８例血标本中２８例阳性，占４８．３％；４例扩张型心肌病血标本中３例阳性，占７５％；１例胎儿羊水标本、４例扩张型心肌病心肌活检及２例尸检心肌组织蜡块均为阳性，占１００％。斑点杂交结果进一步证实了ＰＣＲ扩增产物的特异性。此项研究不仅表明病毒性心肌炎及扩张型心肌病与病毒感染密切相关，而且表明ＰＣＲ技术可以作为临床病毒性疾病早期进行基因诊断的一种新的有效的检测手段。AbstractUsing polymerase chain reaction (PCR), wescreened biopsy and blood specimens taken from 4cases of clinically diagnosed dilated cardiomyopathy,autopsy material of 2 cases and one amniotic fluidspecimen with clinically suspected myocarditis, bloodspecimens taken from 58 cases with clinically sus-pected myocarditis for human picornavirusesinfection. The results demonstrated picornavirus se-quences in biopsies of 4 cases with dilatedcardiomyopathy and blood of 4 cases withmyocarditis, autopsy material of 2 cases, blood speci-mens of 28 cases and one amniotic fluid specimen withclinically suslpected myocarditis. Their positive rateswere 100%, 75%, 100%, 48.3% and 100%respectively. The specific gene amplification productswere identified by dot blot hybridization. This studysuggests that PCR gene amplification provides a newmeans for molecular diagnosis of viral diseases in cli-nical pathology.

关 键 词：心肌病 病毒 RNA 聚合酶链反应 

分 类 号：R725.422[医药卫生—儿科]