低氧胁迫下Ca^(2+)、La^(3+)和EGTA对网纹甜瓜幼苗活性氧代谢的影响  

Effect of Ca^(2+) ,La^(3+) and EGTA on reactive oxygen species metabolism in muskmelon seedlings under hypoxia stress

作　　者：高洪波[1] 郭世荣[1] 刘艳红[1] 汪天[1] 孙艳军[1] 胡晓辉[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095

出　　处：《南京农业大学学报》2005年第2期17-21,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金资助项目(30170645);江苏省自然科学基金(BK2002110);南京市科技计划项目(2003ZB0201)

摘　　要：在根际低氧胁迫下,采用喷叶处理的方法,研究了外源Ca2+及其质膜Ca2+通道抑制剂La3+和Ca2+螯合剂EGTA对网纹甜瓜幼苗根系和叶片抗氧化酶活性、H2O2和MDA含量以及产生速率的影响。结果表明,外源喷施Ca2+处理显著提高了幼苗根系和叶片SOD、POD、CAT活性,降低了H2O2、MDA含量和的产生速率,提高了幼苗的耐低氧能力;而叶面喷施La3+和EGTA处理,幼苗SOD、POD、CAT活性显著降低,加重了活性氧的积累和膜脂过氧化,幼苗耐低氧能力下降。Effect of exogenous foliar spraying Ca 2+ ,plasma membrane Ca 2+ channel blockers La 3+ and Ca 2+ chelator EGTA on reactive oxygen species(ROS)contents and antioxidant enzymes activities in muskmelon seedlings was studied under root-zone hypoxia stress.The results showed that the activities of SOD,POD,CAT in roots and leaves treated by Ca 2+ were obviously higher than those of treated by hypoxia stress,but the contents of H2O2,MDA and production rate of were lower compared with treated by hypoxia stress,which induced higher hypoxia tolerance of muskmelon seedlings.Under the hypoxia stress,5 mmol·L -1 La 3+ and EGTA treatment remarkably inhibited the activities of CAT,SOD,POD,and aggravated the accumulation of ROS and membrane lipid peroxidation of muskmelon seedlings,which led to a lower hypoxia tolerance of seedlings.

关 键 词：网纹甜瓜 CA^2+ 低氧胁迫 抗氧化酶 活性氧 

分 类 号：S641.2[农业科学—蔬菜学]