镉还原法测定血清NO稳定代谢产物NO_3^-/NO_2^-  

CADMIUM REDUCTION METHOD FOR DETERMINATION OF METABOLITES OFNITRATE AND NITRITE FROM NITROGEN OXIDES IN SERUM

作　　者：汪显阳[1] 赵慧卿[1] 

机构地区：[1]安徽医科大学基础医学院,合肥230032

出　　处：《理化检验（化学分册）》2005年第6期417-419,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：安徽省教育厅自然科学基金资助课题(No2003kj222);安徽医科大学优秀中青年骨干教师基金资助课题。

摘　　要：建立了血清NO的间接测定方法。采用正交设计法[L9(34)]观察不同还原剂及其用量、还原时间和温度对NO3-还原率的影响。最佳还原条件为400mg镀铜镉粒室温还原120min,还原率达97%以上。方法简便易行,不需昂贵设备,准确度较高,适合一般实验室用于各种生物医学实验中NO的间接测定。An indirect method for the determination of nitrogen oxides in serum via metabolites nitrate and nitrite was established in this paper. An orthogonal design L_9(3~4) was used to optimise the pasameters such as form and amount of the Cd-reductant, reduction time and reduction temperature. The maximum reduction sate 97% can be obtained under conditions that 400 mg copper-plating cadmium grains was used for reduction at soom temperature over 120 min.

关 键 词：氮氧化物 硝酸盐 亚硝酸盐 镉 血清 

分 类 号：O65[理学—分析化学]