纤维素硫酸钠的相对分子质量与其抗凝血活性的构效关系研究  

STUDY ON RELATIONSHIP BETWEEN MOLECULAR WEIGHT OF SODIUM SULFATE-CELLULOSE AND ITS ANTICOAGULATION ACTIVITY

作　　者：王兆梅[1] 李琳[1] 陈玲[1] 何传波[1] 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510640

出　　处：《林产化学与工业》2005年第2期31-34,共4页Chemistry and Industry of Forest Products

基　　金：广东省自然科学基金资助项目(000453)

摘　　要：纤维素硫酸钠(Na-CS)具有突出的抗凝血活性,为了研究相对分子质量(Mw)与其抗凝血活性的构效关系,需要制备具有不同Mw、硫酸酯取代度相同的Na-CS系列类似物。本实验通过酸水解和凝胶过滤-离子交换联合分离的方法获得不同Mw的Na-CS样品,以此为研究对象,考察了Na-CS的M对其抗凝血效果和对凝血因子抑制作用的影响规律。结果表明,在一定范围内,随着Mw增大,Na-CS对活化部分凝血活酶时间(APPT)和凝血酶时间(TT)的延长作用增强,对凝血因子Ⅱa和Xa的抑制作用提高。Sodium sulfate-cellulose (Na-CS) had high anticoagulation activity. In order to study the relationship between the molecular weights ( M _w) and the anticoagulation activity of Na-CS, it was essential to prepare a series of Na-CS analogues having different M _w with the same sulfate substitution degree. In this study, Na-CS samples having different M _w were obtained by combinated separation method of acid hydrolysis and gel filtration-ion exchange. With these samples as objects of study, the effects of M _w on the anticoagulation efficacy of Na-CS and the inhibition of coagulation factors Ⅱa and Xa were studied. It was indicated that, with the increasing of M _w of Na-CS in a certain range, the prolongation on activated partial thrombo-plastin time (APTT) and thrombin time (TT) was increased and the inhibition on IIa and Xa was improved.

关 键 词：纤维素硫酸钠 抗凝血 凝胶过滤 离子交换分离 

分 类 号：TQ423[化学工程]