早熟染色体凝集技术研究~3H-TdR掺入CHL细胞染色体的损伤  

Study of chromosome damage induced with^3H-TdR β-ray irradiation by PCC technique

作　　者：杨新海[1] 饶用清[1] 

机构地区：[1]北京医科大学基础医学院放射医学教研室

出　　处：《中华放射医学与防护杂志》1994年第1期25-28,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：作者采用ＰＣＣ技术研究了３Ｈ－ＴｄＲ掺入受β射线照射的体外培养ＣＨＬ细胞染色体的损伤．实验结果表明，随着加氚活度增加染色体损伤逐渐增多，量效关系符合二次多项式Ｙ＝ａ＋ｂＤ＋ｃＤ２，对Ｇ１和Ｇ２ＭＰＣＣ断片分别拟合亦是如此．用ＰＣＣ技术对损伤的检出率比相同条件下用常规法的检出率高６～１５倍，说明ＰＣＣ技术远较常规法敏感，其结果更能反映染色体的初始损伤情况，这对客观评价３Ｈ－ＴｄＲ所致的遗传效应具有重要意义．The cytogenetic effect of 3H-TdR β-ray internal radiation on CHL cells was investigated simultaneously by premature chromosome condensation (PCC) and c onventional chromosome alteration analysis (CCAA). The experimental results indicated that the dose response relationships for total chromosome aberrations and G1PCC or G2PCC damage could be accurately expressed by a quadratic equation. But the yield of chromosome fragments observed by PCC was 6-15 times higher than that by CCAA .This fact suggests that PCC is more sensitive than CCAA for determining the chromosome damage. PCC technique might be more useful for assessment of the initial damage of chromosomes.

关 键 词：Β射线 照射 PCC技术 染色体损伤 

分 类 号：R818.74[医药卫生—放射医学]