重组人白细胞介素-6对照射小鼠早期造血功能恢复的影响  

Effect of rhIL-6 on early hematopoietic recovery of irradiated mice

作　　者：邱丽玲[1] 柳晓兰[1] 赵振虎[1] 谈震宇 张卿西[1] 王嘉玺[1] 凌世淦[1] 

机构地区：[1]北京放射医学研究所,北京基础医学研究所

出　　处：《中华放射医学与防护杂志》1994年第4期225-228,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：作者报道了国内重组人白介素－６（ｒｈＩＬ－６）对６．５Ｇｙ照射Ｃｓ７ＢＬ／６Ｊ小鼠后１０天造血功能（主要是造血干细胞、粒系和红系祖细胞）恢复的影响．结果表明；ｒｈＩＬ－６对照后早期造血的影响与白细胞介素－６（ＩＬ－６）剂量有关，且作用是双向的．当ＩＬ－６剂量在２００～１０００μｇ·ｋｇ－１·ｄ－１时，脾重和内源性肿结节（ＣＦＵ－Ｓ）数随ＩＬ－６剂量增大而逐渐增加，与对照组相比，ＣＦＵ－Ｓ数的增加统计学意义更明显．与此同时骨髓有核细胞数和ＣＦＵ－ＧＭ产率以及ＣＦＵ－ＧＭ／股骨也明显增加，但１０００μｇ·ｋｇ－１·ｄ－１组的效果反而下降，因此，ｒｈＩＬ－６促进照后早期造血恢复的最佳剂量可能在５００μｇ·ｋｇ－１·ｄ－１左右．当ＩＬ－６剂量为１０μｇ·ｋｇ－１·ｄ－１时，对照射小鼠造血恢复起抑制作用。这些发现对ｒｈＩＬ－６的应用是很有实际意义的．The effect of recoillbinant human interleukin-6 (rhIL-6 ) on the early phase of postirradiation hematopoietic rccovery (mainly CFU--S, CFUGM and CFU--E ) was studied in 6. 5 Gy γ-irradiated C57 BL/6 J mice after subcutaneoiis injection of rhIL-6twice daily at 10, 100, 200, 500 and 1000 μg/kg/d for 4 or 6 consecutive days started at 30 min after irradiation.The results showed that the effect of IL--3 on the early phase of postirradiation hematopoietic recovery was dose--dependellt and diphasic.rhIL--6 at the doses of 2001000 μg/kg/d had a favorable effect on post-- irradiation hematopoietic recovery expressed as an increase in spleen weight and CFU-S and an eleyation of CFU-GM yield and total content in femur. Administration of relatively low doses of IL -6(10-100μg/kg/d) suppressed postirradiation hematopoietic recovery. Too high a dose of IL-6 (1000μg/kg/d)was not necessary to show a stronger stimulating effect on postirradiation hematopoietic reconstitution.There was an optimal range of IL--6 dose for treating the radiation-induced bone marrow failure, which lay near 500μg/kg/d.These findings are of practical value to the application of rhIL--6.

关 键 词：白细胞介素6 RHIL-6 照射 早期 

分 类 号：R818.059[医药卫生—放射医学]