重组人干细胞因子对放射病患者造血细胞的体外作用  

In vitro effects of recombinant human stem cetll factor on hematopoietic cells from patients with acute radiation sickness

作　　者：李川晟[1] 程涛[1] 许燕群[1] 

机构地区：[1]上海第二军医大学长海医院血液科

出　　处：《中华放射医学与防护杂志》1994年第4期221-224,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：本文作者报道了用体外半固体培养方法测试重组人干细胞因子、重组人粒－单系集落刺激因子、重组人白细胞介素－３和重组人粒－单系集落刺激因子／白细胞介素－３的融合蛋白等４种造血生长因子对５例骨髓型急性放射病人远期造血细胞体外生长的作用．结果显示；用重组人干细胞因子对正常骨髓最佳刺激浓度为５×１０４ｎｇ／Ｌ的４例病人有不同程度的正向刺激作用，１例病人呈抑制作用，对重组人干细胞因子反应不均一性与受照剂量有关．细胞表面抗原和细胞形态，组化测定，重组人干细胞因子在本文体系中主要促进粒－单系集落生长．重组人干细胞因子与其它３种造血生长因子伍用可增加集落产率增大集落体积．作者对融合蛋白也作了比较分析，认为融合蛋白是一种非常有价值新的造血生长因子．The effects of rhSCF, rhPIXY 321,rhGM-CSF and rhIL-3 on clanal proliferation of hematopoietic cells from five. cases of acute radiation sickness. were studied. The results showed that rhSCF could stimulate clodal proliferation of normals hematopoietic eells and the best results were obtained when the concentration of rhSCF was 5 ×104ng/L. Clonal proliferation of hematopoietic cells from four cases of acute radiation sickness was stimulated while that from one case was inhibited. Moreover, the responsiveness of cells to rhSCF was correlated with the doses of radiation. Analysis of cell surface antigen, cell morphology and histochemistry revealed that rhSCF promoted predominantly the proliferation of granulocyte--macrophagg lineage. rhSCF in eombination faith other three factors could further enhance the clonal proliferation of hernatopbiriefic cells. Finally, we have also analysgd the effects of rhPIXY 321, a fusion protein of GM-CSF and IL-3 and found it to be a novel valuable hematopoietic growth factor.

关 键 词：干细胞因子 造血生长因子 放射病 

分 类 号：R818.05[医药卫生—放射医学]