低切应力作用下体外培养动脉的内皮素表达  

EXPRESSION OF ENDOTHELIN IN ORGAN-CULTURED ARTERY UNDER LOW SHEAR STRESS

作　　者：刘波[1] 周瑾[2] 刘艳春[3] 滕海英[4] 姜宗来[1] 

机构地区：[1]上海交通大学医学院力学生物学与医学工程实验室,上海200030 [2]第二军医大学生物化学教研室,上海200433 [3]第二军医大学解剖学教研室,上海200433 [4]第二军医大学数理教研室,上海200433

出　　处：《生物物理学报》2005年第3期200-204,共5页Acta Biophysica Sinica

基　　金：国家自然科学基金资助项目(10132020;30100060)

摘　　要：研究了切应力对完整血管的生物学作用以及应力引起血管重建过程中内皮素(ET)的变化。采用血管体外应力培养系统,将一段完整的猪颈总动脉在体外进行培养,设切应力分别为2Pa(S20组)和0.5Pa(S5组),设置2、4、6、8、10、12、14、16和18h共9个时相观察点,非平衡法放射免疫检测灌流液中的ET含量。通过Logistic曲线方程拟合,分析切应力作用下完整动脉ET表达变化规律。结果显示:S20组ET总体变化不明显;S5组分泌速率在前12.37h内明显上升,而后又逐渐下降趋于稳定,且始终高于S20组。说明低切应力作用下ET的表达及分泌增高。结果提示,在低切应力引起的血管重建中,ET可能起着重要作用。The biological effect of shear strees on intact artery and the change of Endothelin (ET) during the stress-induced vascular remodeling was studied. The common carotid arteries of pigs were cultured with a artery organ-culture system under different shear stress, 0.5 and 2 Pa respectively, 1.33×10-4 Pa of pressure and stable flow in vitro. Cultured for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h, the content of ET in the perfusate was examined with radio-immunity method, and the data were described by Logistic equation. The secretion velocity of ET didn't change obviously in S20 group, while in S5 group it rised evidently at the first 12 h and then descend to a stable level which was higher than S20 group all the time. The secretion and expression of ET rised under low shear stress. It may play an important role in vascular remodeling induced by low shear stress.

关 键 词：切应力 颈总动脉 血管内皮细胞 内皮素 

分 类 号：R318.01[医药卫生—生物医学工程] R329.27[医药卫生—基础医学]