系统性白色念珠菌感染小鼠动物模型的研究  

Research of C57BL/6 mouse model of systemic Candida albicans infection

作　　者：杨琼[1] 宋祥福[2] 王桂云[1] 

机构地区：[1]东北师范大学遗传与细胞研究所,吉林长春130024 [2]吉林大学公共卫生学院,吉林长春130021

出　　处：《东北师大学报（自然科学版）》2005年第2期86-89,共4页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30040014);吉林省科技厅资助项目(20010416)

摘　　要：　为了建立稳定的系统性白色念珠菌感染的C57BL/6小鼠模型,选用国际标准型白色念珠菌10231(Candidaalbicans),经小鼠尾静脉直接注射白色念珠菌酵母细胞;接种后从感染小鼠的脾中能够分离出白色念珠菌,病理学检查肾组织可见肾盂内有大量白色念珠菌的孢子、菌丝体和炎性细胞浸润.噬菌体展示的白色念珠菌HSP90多肽和热灭活的白色念珠菌菌苗能明显减少感染小鼠肾脏负荷的真菌量.结果说明经尾静脉注射合适剂量的白色念珠菌可建立稳定的小鼠系统性白色念珠菌感染模型,该模型可用于研究系统性白色念珠菌感染的致病机理、免疫和预防.<Abstrcat>To establish a stable C57BL/6 mouse model of systemic candidiasis, the mice were challenged intravenously via the lateral tail vein with 2 × 107 or 106 C.albicans yeast cells, respectively. And then the organs of mice were taken out to examine the C. albicans and pathology. The results showed that C. albicans could be isolated from the spleen of all the mice and the histologic section of kidney exhibited C. albicans blastospores and hyphae, and abundant inflammatory cells. Mice immunized with phage displayed peptide and heat killed C. albicans acquired a resistance to systemic C. albicans infection as confirmed by fewer C. albicans cells in the kidneys. These results suggest that the C57BL/6 mouse model of systemic candidosis has been established stably when a suitable does of C. albicans was intravenously given to mice. The model may be used for studying the prevention and cure of systemic C. albicans infection.

关 键 词：白色念珠菌 感染 动物模型 

分 类 号：R392.3[医药卫生—免疫学]