大叶柴胡愈伤组织继代培养及其RAPD分析  

Long-term subculture of calli and RAPD analysis in Bupleurum longiradiatum Turcz.

作　　者：付杨[1] 陈凤清[1] 孙冬雪[1] 苏全平[1] 王丽[1] 

机构地区：[1]东北师范大学遗传与细胞研究所,吉林长春130024

出　　处：《东北师大学报（自然科学版）》2005年第2期90-92,共3页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20030551-121)

摘　　要：　以野生的大叶柴胡为材料,通过离体培养诱导产生愈伤组织,利用RAPD分子标记的方法,在DNA水平上分析野生植株和愈伤组织的遗传变异.从13个10碱基引物中筛选出7个引物,对它们的PCR扩增结果进行了分析.结果表明柴胡愈伤组织与野生苗相比出现了DNA水平变异.<Abstrcat>Bupleurum longiradiatumTurcz. were used for inducationg of calli in vitro. Genetic differences between wild plantlets and calli had been analysed on DNA level with the application of random amplified polymorphic DNA(RAPD) technology to the molecular genetic marker. The fingerprints amplification by polymerase chain reaction(PCR) with 7 polymorphic 10-based random primers selected from 13 ones was analysised. The results indicated that there were genetic variation between wild plantlet and calli.

关 键 词：大叶柴胡 愈伤组织 RAPD 

分 类 号：Q943.1[生物学—植物学]