对A_(72)与PK_(15)进行猪传染性胃肠炎血清中和试验的对比观察  

THE OBSERVATION OF A_(72) CELL AND PK_(15) CELL ON THE SERUM NERTRALIZATION TEST OF TRANSMISSIBLE GASTROENTERITIS

作　　者：董志珍[1] 赵祥平[1] 霍蕾[1] 肖妍[1] 

机构地区：[1]天津出入境检验检疫局,天津300457

出　　处：《检验检疫科学》2005年第3期28-29,共2页Inspection and Quarantine Science

摘　　要：〔目的〕建立以狗直肠瘤(A72细胞)进行TGEV血清中和试验的方法。〔方法〕引进A72细胞系,建立成熟培养环境;以库存94份猪血清观察TGEV在A72与PK15中的增殖与病变,并采用ELISA试验进行验证。〔结果〕以A72细胞培养增殖的TGEV毒价高于以PK15细胞培养增殖的TGEV毒价。在血清中和试验中A72病变明显,易于观察,较PK15细胞的人为误差小。〔结论〕A72细胞可以准确清晰地反应TGEV感染,其敏感性和准确性优于PK15细胞。Object:To establish the method of serum neutralization test of transmissible gastroenteritis by using A 72 cell.Method:1.Introduce A 72 cell line and build an excellent culture condition.2.Study the proliferation and pathological change of transmissible gastroenteritis virus of swine in A 72 cell and PK 15 cell by observing 94 pig serum and verify it by using enzyme-linked immunosorbent assay.Result:1.The titer of transmissible gastroenteritis virus of swine is higher in A 72 cell than in PK 15 cell.2.The pathological changes of A 72 cell are more clear than PK 15 cell in the serum neutralization test.Conclusion:A 72 cell can reflect the infection of transmissible gastroenteritis virus of swinse correctly and is sensitvity and correctness is better than PK15 cell.

关 键 词：血清中和试验 猪传染性胃肠炎 对比观察 PK15细胞 ELISA试验 TGEV 细胞培养 培养环境 人为误差 细胞系 增殖 猪血清 准确性 敏感性 病变 毒价 狗 

分 类 号：S858.285.3[农业科学—临床兽医学] S858.286.4[农业科学—兽医学]