毛细管电泳-电致化学发光法测定人尿中脯氨酸和羟脯氨酸  

Determination of Proline and Hydroxyproline in Human Urine with Capillary Electrophoresis-Electrochemiluminescence

作　　者：薛静[1] 梁恒[1] 李甜[1] 武亚艳[1] 

机构地区：[1]西安交通大学生物医学信息工程教育部重点实验室,分离科学研究所,西安710049

出　　处：《分析化学》2005年第6期785-788,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(No.20299030;20175015)

摘　　要：采用毛细管电泳联吡啶钌电致化学发光法检测了人尿中的脯氨酸和羟脯氨酸。讨论了联吡啶钌浓度、检测电位、磷酸盐缓冲液浓度和pH值、进样电压和进样时间等实验条件对脯氨酸和羟脯氨酸分离检测的影响。在优化实验条件下,脯氨酸和羟脯氨酸分别在0.008～2和0.01～2mmol/L范围内呈良好线性(相关系数0.997);检出限浓度分别为2和4μmol/L。测定了人尿中游离脯氨酸和羟脯氨酸以及两者总浓度,回收率96.4%～99.7%。A method of capillary electrophoresis (CE) with tris (2,2'-bipyridyl) ruthenium ( H) electrochemiluminescence (ECL) detection has been applied to the simultaneous determination of proline (Pro) and hydroxyproline (Hyp) in human urine. Under optimized conditions, such as detection potential at 1.18 V, electrokinetic injection at 10 kV for 10 s, seperation voltage at 20 kV, 10 mmol/L seperation buffer with pH 8.5, 5 mmol/L ru(bpy)(3)(2+) and 50 mmol/L phosphate buffer with pH 7.0 in the ECL cell, the linear ranges were 0.008 similar to 2 mmol/L for Pro with a correlation coefficient of 0. 997, and 0. 01 similar to 2 mmol/L for Hyp with a correlation efficient of 0.998. Detection limits for Pro and Hyp were 2 and 4 mu mol/L respectively. When the method was applied to determine free and total Pro and Hyp in human urine, the recoveries were between 96.4% and 99.7%, respectively.

关 键 词：羟脯氨酸 化学发光法 毛细管电泳 人尿 测定 磷酸盐缓冲液 联吡啶钌 实验条件 游离脯氨酸 分离检测 进样时间 相关系数 pH值 MOL 检出限 总浓度 回收率 电位 

分 类 号：R446.12[医药卫生—诊断学]