透明颤菌血红蛋白基因(vgb)表达载体的构建及其诱导表达  

The construction and inducing expression of veb expression vector

作　　者：吴迪[1] 杨明明 张亚妮[1] 张炜炜 周乍琴[1] 樊俊华 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]武汉阳光广济医药开发有限公司,湖北武汉430072

出　　处：《黑龙江畜牧兽医》2005年第6期6-8,共3页Heilongjiang Animal Science And veterinary Medicine

摘　　要：利用PCR技术将透明颤菌血红蛋白基因(vgb)克隆到融合表达载体pET28a,在大肠杆菌BL21(DE3)表达。重组蛋白分别在30℃和37℃诱导表达,30℃诱导获得可溶性表达。结果表明:在30℃,1.5mmol/L和2.5mmol/LIPTG诱导下,诱导4～8h透明颤菌血红蛋白可获得高表达透明颤菌血红蛋白的表达量分别占菌体总蛋白的18.7%和27.7%。The vgb gene was cloned to the soluble expression vector PET28a,was expressed in E.coli BL21.The reconstructive protein was induced at 30 ℃and 37℃,respectively. The soluble expression was obtained at 30 ℃.The result showed: the vgb obtained high expression by induced four to eight hours at 30℃,using 1.5 mmol/L or 2.5 mmol/L IPTG-the expression products of veb accounted 18.7% and 27.7% at all protein.

关 键 词：透明颤菌血红蛋白基因 诱导表达 表达载体 构建 PCR技术 可溶性表达 融合表达 大肠杆菌 重组蛋白 总蛋白 表达量 高表达 克隆 

分 类 号：Q78[生物学—分子生物学] Q786