石歧杂鸡胚胎成纤维细胞的体外培养  

The fibroblast culture of Shiqiza-chicken embryo in vitro

作　　者：魏亚茹[1] 刘娣 关伟军[2] 李景芬[1] 

机构地区：[1]东北农业大学动物科技学院,黑龙江哈尔滨150030 [2]中国农业科学院畜牧研究所,北京100094

出　　处：《黑龙江畜牧兽医》2005年第6期10-12,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家基础性工作重大专项(2001DEA10006);国家自然科学基金项目资助(30100132).

摘　　要：用组织块法对石歧杂鸡胚胎成纤维细胞进行原代和传代培养,探讨禽类胚胎成纤维细胞适宜的培养模式。成功获得较均一的稳定的细胞群体,并成功地对细胞进行了冷冻保存和复苏。对于进行大规模的动物细胞培养具有重要的指导意义。Shiqiza-chicken embryo fibroblasts were primary and sub-cultured by tissue method,investigating the culture model of poultry embryo fibroblasts.The results indicated that tissue method could obtain more homogeneous and stable cell population,and cells-freezing and thawing were successful.It is important to culture a great amount of animal cell.

关 键 词：胚胎成纤维细胞 石歧杂鸡 体外培养 动物细胞培养 传代培养 培养模式 细胞群体 冷冻保存 禽类 

分 类 号：S865.13[农业科学—野生动物驯养] S831[农业科学—畜牧兽医]