不同细胞因子组合对脐带血中ACl33^(+)细胞迁移和归巢能力的影响  

Effects of Different Cytokine Combinations on the Migration and Homing Capabilities of Umbilical Cord Blood AC133^(+)Cells

作　　者：戴宏[1] 刘玉龙[1] 姜忠[1] 周丽英[1] 胡勤芳[2] 郭晓葵[1] 周剑影[1] 

机构地区：[1]苏州大学附属第二医院,中国核工业辐射损伤医学应急中心,江苏苏州215004 [2]北京核工业医院,北京100081

出　　处：《苏州大学学报（医学版）》2005年第3期351-354,共4页Suzhou University Journal of Medical Science

基　　金：国防科工委"十五"国防预研项目资助课题(Y-020403-QS)

摘　　要：目的探讨不同细胞因子组合对脐带血中ACl33+细胞表面趋化因子受体(CXCR4)、粘附分子VLA4(CD49d)、LFA1(CD11a)和Lselectin(CD62L)表达的影响。方法采用直接免疫荧光标记法,使用流式细胞仪测定新鲜分离的、不同培养条件下体外培养7d、14d的脐带血中ACl33+细胞表面CXCR4和CD49d、CD11a和CD62L的表达情况。结果新鲜脐带血ACl33+细胞中CXCR4的表达率为(50.2±12.5)%;CD49d的表达率为(53.2±15.6)%,CD11a的表达率为(98.1±2.4)%,CD62L的表达率为(52.4±16.6)%。在无血清、无细胞因子存在的条件下,体外培养第7d及14d,CXCR4、VLA4、CD11a和CD62L的表达显著下降(P<0.05)。在有细胞因子存在的条件下,短期液体扩增培养后,脐带血ACl33+细胞中CXCR4、VLA4、CD11a和CD62L的表达率较新鲜ACl33+细胞均有不同程度的增高(P<0.05);IL3+FL+SCF组较IL11+FL3+SCF组表达率高,其中以培养第14dCXCR4、CD62L的表达增高最明显。结论在体外短期扩增培养过程中,早期效应细胞因子能显著上调脐带血ACl33+细胞CXCR4、CD49d、CD11a和CD62L等的表达;用SCF、FL和IL3组合扩增脐带血,不仅能获得足够数量的造血干/祖细胞,还能增加造血干/祖细胞的迁移和归巢能力。Objective To investigate the effect of different cytokine combinations on the expression of chemokine receptor(CXCR4), VLA4(CD49 d), LFA-1(CD11a) and L-selectin (CD62L) in umbilical cord blood AC133^+ cells.Methods The expression rate of the CXCR4, CD49 d, CD11a and CD62L on the surface of umbilical cord blood AC133^+ cells which were freshly isolated and incubated in serum-free liquid culture system for 7 d and 17 d with different cytokine combinations in vitro were analyzed by flow cytometric two-colour direct immunoflurescence methods. Results The expression rate of the CXCR4, CD49 d, CD11a and CD62L on the surface of fresh umbilical cord blood AC133^+ cells was 50.2%±12.5%, 53.2%±15.6%, 98.1%±2.4 SED and 52.4%±16.6 SED respectively. After short-term incubation in serum-free culture system with no cytokine in vitro at d 7 and d 14, the expression rate remarkably declined. After short-term incubation in serum-free culture system with cytokines in vitro, the expression rate was higher at different levels than that in fresh umbilical cord blood. The expression rate was remarkably higher in the presence of IL-3, FL and SCF combinations than that in the presence of IL-11, FL and SCF combinations. [WTHZ]Conclusions [WTBZ]During short-term cul- ture in vitro, the early-effective cytokines can up-regulate the expression of CXCR4, CD49d, CD11a and CD62L in umbilical cord blood AC133^+ cells. In the presence of IL-3, FL and SCF combinations, we may not only acquire enough hematopoietics stem/progenitor cells, but also gain its migration and homing capabilities.

关 键 词：脐带血 ACl33^(+)细胞 细胞因子 细胞粘附分子 迁移 归巢 

分 类 号：R818.74[医药卫生—放射医学]