实验性大鼠肺纤维化病理形态及超微结构观察  

Study on Pathomorphology and Ultrastructure of Rat Pulmonary Fibrosis Model

作　　者：王响英[1] 吴淑燕[1] 李苏安[1] 毛棣华[1] 

机构地区：[1]苏州大学医学院基础医学系,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2005年第3期379-382,386,共5页Suzhou University Journal of Medical Science

基　　金：香港保健协会基金资助

摘　　要：目的从形态学上研究肺纤维化的形成过程,为防治研究和客观地评价用药物治疗肺纤维化的疗效提供依据。方法向大鼠气管内一次性注入博莱霉素(5mg/kg),观察注药后各模型组的肺系数、肺组织定量分析、肺组织病理及超微结构的变化。结果模型组肺系数从第7d开始呈逐渐升高趋势;定量分析:7d组肺泡炎最严重,28d组肺泡腔明显缩小,胶原纤维成分增加;光镜下7d组肺泡腔及肺泡间质内以炎细胞浸润为主,14d时肺泡间质增宽,成纤维细胞增多,28d时胶原纤维增生,部分肺泡腔萎缩消失;电镜下3d和7d时肺泡上皮线粒体肿胀,空泡化,核固缩或核染色质边集,肺毛细血管基膜水肿或增厚,14d时Ⅱ型肺泡上皮细胞增生,其内板层小体增多,成纤维细胞增多;28d时肺间质增宽,巨噬细胞及成纤维细胞增多,胶原纤维呈灶性沉集。结论建立的大鼠肺纤维化模型符合肺泡炎-纤维化的早期演变过程。Objective By quantitative analysis and evaluating the pathomorphology and ultrastructural changes in various periods of rat pulmonary fibrosis model,the evident for the preventive effect and therapeutic mechanism in treating pulmonary fibrosis are offered.Methods Pulmonary fibrosis was made by non-exposure intratracheal injection of Bleomycin. The pathomorphology and ultrastructure were observed, the weight of lungs and rats were recorded, then the pulmonary index (PI) was evaluated. Results The PI increased during the pulmonary fibrosis progress. The most badly alveolar inflammation occur in at 7th day and the obvious shrink of alveolar cavity and increase of collagen fibers at 28th day were found by means of the quantitative analysis. The infiltrating inflammatory cells and the increase of collagen fibers were able to be seen under the light microscope. The ultrastructure in most of them showed that the collagen fibers in alveolar septum was obviously increased and the honeycomb shape of pulmonary structure destroyed. Type Ⅱ alveolar epithelial cells hyperplasia also be seen. Conclusion The pulmonary fibrosis model induced by Bleomycin is typical in the development of alveolitis to pulmonary fibrosis.

关 键 词：肺纤维化 大鼠 形态定量分析 病理形态 超微结构 

分 类 号：R563.902[医药卫生—呼吸系统]