蛇床子总香豆素对去卵巢大鼠骨质疏松的影响  

The Effect of Total Coumarines From the Fruits of Cnidium Monnieri on Osteoporosis in Ovariectomized Rats

作　　者：鲍君杰[1] 谢梅林[1] 周佳 朱路佳[1] 

机构地区：[1]苏州大学,江苏苏州215007 [2]西安绿泉生物技术有限公司,陕西西安710075

出　　处：《苏州大学学报（医学版）》2005年第3期387-390,共4页Suzhou University Journal of Medical Science

摘　　要：目的观察蛇床子总香豆素对去卵巢大鼠骨质疏松的影响。方法40只大鼠随机分成假手术组、模型组、蛇床子总香豆素20mg/kg组和尼尔雌醇组,对后3组大鼠行卵巢切除术,12周后检测大鼠体重、子宫重量、子宫内膜腺体数及血清钙、磷、碱性磷酸酶、肌肝、E2、CT、TGF-β1、BGP含量和大鼠股骨骨密度及形态改变。结果与模型组相比,蛇床子总香豆素组大鼠体重、子宫重量和子宫内膜腺体数未见有明显增加或降低(P>0.05),而血清钙含量明显增加(P<0.05),血清磷含量下降(P<0.05);蛇床子总香豆素组大鼠血清E2、CT、TGFβ1、BGP含量均显著升高(P<0.01或P<0.05),股骨近端干骺端骨密度明显增加(P<0.01),光镜下可见骨小梁增多、增粗,呈网状,髓腔变小,与假手术组大鼠相似。结论蛇床子总香豆素能提高去卵巢大鼠股骨骨密度,改善股骨组织形态,预防骨质疏松的发生。其作用机制可能与增加血清E2、CT、TGFβ1和BGP含量等相关。Objective To observe the effect of total coumarines from the fruits of Cnidium monnieri(TCFC) on osteoporosis in ovariectomized condition.Methods Forty female SD rats were divided into sham-ovariectomized group (Sham), ovariectomized group(OVX),20 mg/kg TCFC group,1 mg/kg nilestriol group.12 weeks later, body weight, uterus weight,the number of endometrial gland, Ca^(2+), phosphorus, ALP, creatinine, E_2,CT,TGF-β_1, BGP and bone mineral density (BMD) was measured. Then pathomorphology of the tibia of rats was observed. Results There was no significant difference between TCFC group and OVX group in the body weight, uterus weight and the number of endometrial gland(P>0.05).Compared with the group of OVX, in the TCFC group the serum level of phosphorus was lower, Ca^(2+),E_2,CT,TGF-β_1,BGP and BMD were significantly higher (P<0.01 or P<0.05).The bone trabecula was more widened and less broken in TCFC group than that in OVX group. Conclusion TCFC can prevent osteoporosis in overiectomized rats, this may be related with the increasing of E_2,CT TGF-β_1 and BGP.

关 键 词：蛇床子总香豆素 骨质疏松 大鼠 

分 类 号：R285.5[医药卫生—中药学] R591.1[医药卫生—中医学]