普罗布考对兔动脉粥样硬化模型主动脉凝集素样氧化低密度脂蛋白受体-1表达的研究(英文)  

Probucol represses lectinlike oxidized LDL receptor-1 expression in atherosclerotic lesions of NZW hyperlipidemic rabbits

作　　者：龙开超[1] 孙明[1] 

机构地区：[1]中南大学湘雅医院心内科,湖南长沙410008

出　　处：《中国现代医学杂志》2005年第10期1450-1453,共4页China Journal of Modern Medicine

摘　　要：目的观察普罗布考对兔动脉粥样硬化模型高胆固醇血症、主动脉内皮功能及LOX-1表达的影响。方法新西兰大白兔随机分为正常对照组(普通饮食,6只),高脂饮食组(高脂饮食,10只)及普罗布考组(高脂饮食+普罗布考200mg/kg·d,10只)。实验开始前及第12周分别耳缘静脉采血测定血清总胆固醇。第12周处死动物,取胸主动脉,制备离体胸主动脉环对乙酰胆碱的反应以检查内皮功能,RT-PCR与免疫印迹检测凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因与蛋白质表达水平。结果普罗布考可以显著降低兔动脉粥样硬化模型血清总胆固醇浓度;防止高脂血症兔胸主动脉对乙酰胆碱引起的内皮信赖性舒张(E-DR)反应明显降低;以及下调模型动脉主动脉明显增强了LOX-1表达。结论兔动脉粥样硬化模型内皮功能损伤的机制与LOX-1表达增强有关,普罗布考保护内皮功能可能的机制是下调LOX-1表达的作用。[Objective] The effects of probucol on total cholesterol concentrati on, endothelium-dependent vasodilator activity and repression of Lectinlike Oxid ized Low Density Lipoprotein Receptor-1(LOX-1) expression were tested in a NZW r abbit atherosclerosis model. [Methods] Twenty-six NZW rabbits were randomly di vided into three groups: a normal control group (fed on normal commercial rabbit diet, n =6), a hyperlipidemic group (fed on high-fat diet, n =10) and a probuco l treatment group (fed on high-fat diet and probucol 200 mg/kg·d, treatment n = 10). Plasma total cholesterol (TC) concentration at the beginning and the 12-wee k end point of the experiment determined, and all the experimental rabbits were sacrificed, and endothelial function test were performed on thoracic aortic ring s prepared from the isolated aorta using the vascular endothelium-dependent rela xation (EDR) parameter in response to acetylcholine The aortic atherosclerotic l esion expression of LOX-1 mRNA and protein were examined by RT-PCR and Western b lotting respectively. [Results] Probucol significantly lowered plasma TC level , protected endothelial function as indicated by the improvement of vascular EDR of aortic rings in response to acetylcholine, and repressed the LOX-1 mRNA and protein expression that were over-expressed in the atherosclerosis models. [Con clusion] Over-expression of LOX-1 might be one of the mechanism of endothelial dysfunction in atherosclerotic model and probucol repression of LOX-1 plays an i mportant role in improvement of endothelial function.

关 键 词：普罗布考 动脉粥样硬化 凝集素样氧化低密度脂蛋白受体-1 内皮功能 

分 类 号：R972.6[医药卫生—药品]