表达CpTI或CpTI+Bt基因水稻对鳞翅目害虫抗性的田间评价  

Field evaluation of the resistance of transgenic rice expressing CpTI or CpTI+ Bt to lepidopterous pests

作　　者：李玥仁[1] 胡奇勇[2] 郑宇[2] 胡习斌[2] 张晓俊[2] 李本金[2] 

机构地区：[1]福建省农科院中心实验室 [2]福建省农科院植物保护研究所,福建福州350003

出　　处：《福建农林大学学报（自然科学版）》2005年第2期181-184,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30170636);福建省自然科学基金资助项目(B0020003)

摘　　要：结果表明,表达CpTI或CpTI+Bt基因水稻可在整个生育期对鳞翅目害虫保持高度或中度抗性.在分蘖期和灌浆期,相应普通水稻已遭受钻蛀性鳞翅目害虫(包括二化螟、三化螟和大螟等)严重危害,枯心率或白穗率均达10%以上;而该转基因水稻在分蘖期和灌浆期的枯心率或白穗率比对照分别下降了69.80%-96.40%和83.40%-93.30%.田间调查表明,相应普通水稻稻叶平均受害指数高达23.52时,转基因水稻稻叶受害指数仅为0.37-4.05,该转基因水稻可有效控制稻纵卷叶螟为害.但该转基因水稻对褐飞虱、电光叶蝉和黑尾叶蝉的抗性与普通水稻未见明显差异.The results showed that the transgenic rice expressing CpTI or CpTI+ Bt had high or moderate resistance to lepidopterous pests in the whole growing season. The control effic acies of the transgenic rice against stem borers, including Chilo suppressalis , Tryporyza incertalas, and Sesamia inferens, were up to 69.80%-96. 40% at tillerin g stage and 83.40%-93.30% at filling stage, while percentage of “dead heart” at tillering stage and percentage of “white head” at filling stage were above 10 % for corresponding non-transgenic rice. The field investigation also indicated that the transgenic rice could control the population of Cnaphalo crocis medinulsis in paddy fields effectively. The average leaf damage indexes of the transgenic rice ranged from 0.37 to 4.05, in comparison with 23.52 for the corresponding n on-transgenic rice. However, there was no significant difference in resistance to Nilaparvata lugens, Reiclia dorsalis and Nephotettix cincticeps betwe e n the transgenic rice and their corresponding non-transgenic rice.

关 键 词：Bt基因 CPTI 田间评价 害虫抗性 表达 转基因水稻 鳞翅目害虫 受害指数 稻纵卷叶螟 有效控制 黑尾叶蝉 电光叶蝉 灌浆期 分蘖期 生育期 二化螟 三化螟 调查表 褐飞虱 白穗 心率 大螟 为害 

分 类 号：S562[农业科学—作物学] S188