雌、孕激素对子宫内膜癌细胞孤儿受体ERRα的调控作用  

Regulation of orphan receptor ERRα by estrogen and progesterone in endometrial carcinoma cell line

作　　者：高敏[1] 魏丽惠[1] 孙蓬明[1] 王建六[1] 赵丹[1] 赵超[1] 王志启[1] 

机构地区：[1]北京大学人民医院妇科,北京100044

出　　处：《北京大学学报（医学版）》2005年第3期281-283,共3页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30371477)资助课题~~

摘　　要：目的:探讨孤儿受体ERRα与雌激素(E)、孕激素(P)之间的相互关系及其在子宫内膜癌发病中的作用。方法:采用逆转录聚合酶链反应(RT PCR)方法,检测不同浓度(10-10, 10-8, 10-6 mol/L)17β雌二醇(17βE2 )作用于Ishikawa细胞系不同时间(0min, 15min, 30min和24h)后ERRαmRNA的变化,并应用ER拮抗剂ICI182780同时作用细胞,观察是否可阻断E2 对ERRα的调控作用;检测不同浓度孕酮( 10-8, 10-7, 10-6, 10-5 mol/L)作用于Ishikawa细胞系24h后ERRαmRNA的变化。结果: 10-10 mol/L17βE2 作用于Ishikawa细胞系15min, 30min及24h后ERRαmRNA水平与未加E2 相比均稍有增加,而10-8, 10-6 mol/L17βE2 作用于细胞系15min, 30min及24h后ERRαmRNA明显减小,以10-8 mol/L作用后减少最明显。同时加入E2 和ICI182780作用于细胞系后,E2对ERRαmRNA的减小作用被阻断。10-8mol/L孕酮作用于细胞系24h后,ERRαmRNA与对照组相比无明显变化,但加入10-7, 10-6和10-5 mol/L孕酮后,ERRαmRNA表达均出现明显增加。结论: 17βE2 可减少子宫内膜癌细胞系Ishikawa细胞ERRαmRNA的表达,此减少作用是通过ER介导完成的。孕酮可增加ERRαmRNA的表达。Objective:To explore the relationship between expression of ERRα mRNA and estrogen and progesterone and to elucidate the function of ERRα in endometrial carcinoma. Methods:The expression of ERRα mRNA was examined by reverse transcription polymerase chain reaction. Endometrial carcinoma cell line Ishikawa was dealt with different concentrations of 17β-estradiol(10 -10 mol/L,10 -8 mol/L and 10 -6 mol/L) for 15 min,30 min and 24 h and 17β-estradiol and ER inhibitor-ICI182780 were given concomitantly to observe the change of ERRα mRNA. Different concentrations of progesterone (10 -8 mol/L,10 -7 mol/L,10 -6 mol/L and 10 -5 mol/L)were also given to Ishikawa cell line for 24 h to observe the change of ERRα mRNA. Results:The expression level of ERRα mRNA was slightly higher than that of the control group after being stimulated for 15 min, 30 min and 24 h by 10 -10 mol/L 17β-estradiol. However the expression level of ERRα mRNA was significantly lower than that of the control group after being stimulated for 15 min, 30 min and 24 h by 10 -8 mol/L and 10 -6 mol/L of 17β-estradiol. When 10 -6 mol/L of ICI182780 and 10 -8 mol/L of 17β-estradiol were given simultaneously to Ishikawa cell line, this down-regulation was blocked. After being stimulated for 24 h by 10 -7 mol/L,10 -6 mol/L and 10 -5 mol/L of progesterone, the expression levels of ERRα mRNA were significantly higher than that of the control group, but no change was found in 10 -8 mol/L of progesterone. Conclusion:17β-estradiol can down-regulate the expression of ERRα mRNA and this regulation is mediated by estrogen receptor. Progesterone can up-regulate the expression of ERRα mRNA.

关 键 词：ERR 调控作用 孤儿受体 孕激素 ICI182780 逆转录聚合酶链反应 ΑMRNA mol/L 17Β-E2 17β-雌二醇 MRNA水平 mRNA表达 不同浓度 子宫内膜癌 24h 相互关系 PCR) 不同时间 癌细胞系 孕酮 雌激素 拮抗剂 对照组 A细胞 检测 

分 类 号：R197.38[医药卫生—卫生事业管理] R962[医药卫生—公共卫生与预防医学]