龙葵总提取物对多发性骨髓瘤U266细胞株的作用  

An in vitro study of cytotoxic and antineoplastic effect of Solanum nigrum L extract on U266

作　　者：王蔚[1] 陆道培[1] 

机构地区：[1]北京大学血液病研究所,人民医院血液科,北京100044

出　　处：《北京大学学报（医学版）》2005年第3期240-244,共5页Journal of Peking University:Health Sciences

摘　　要：目的:研究龙葵总提取物对U266细胞的细胞毒作用及其机制。方法:U266细胞株与龙葵总提取物共同培养,用CCK 8试剂检测细胞毒作用;用流式细胞仪测定细胞周期及凋亡。结果:龙葵总提取物对U266细胞有细胞毒作用,半数抑制(质量)浓度约为117mg/L;龙葵总提取物影响U266细胞周期,G0 /G1 期细胞减少,S期、G2 /M期细胞增加,凋亡细胞增加。用AnnexinV/PI染色及TFAR19染色流式细胞仪检测,均显示细胞凋亡与龙葵总提取物有剂量依赖关系。结论:龙葵总提取物对U266细胞有体外细胞毒作用,其作用机制部分是诱导细胞凋亡。Objective: To study cytotoxicity and antineoplastic effect in vitro Solanum nigrum L extract on U266. Methods: U266 cells were cultured together with the extract of Solanum nigrum L. Cytotoxicity assay was tested by CCK-8. Cell cycle and apoptosis were determined using flow cytometry (FCM) analysis. Results: Extract of Solanum nigrum L showed strong cytotoxicity against U266 cells. The IC_ 50 was about 117 mg/L. After exposure of U266 cells to the drug for 48 hours, the cell cycle distribution was changed compared with the controls. There was decrease of cells in the G_0/G_1 phase with increase of cells in the S phase and G_2/M phase. Apoptosis of U266 cells could be shown with staining of Annexin V FITC/PI or TFAR19 testing through FCM. The proportion of apoptotic cells increased in parallel with the increase of the drug dosage. Conclusion: Solanum nigrum L extract showed strong cytotoxicity effect on U266 cells. The antineoplastic effect of the drug can partly be ascribed to its apoptotic inducing effect.

关 键 词：总提取物 多发性骨髓瘤 细胞株 龙葵 U266细胞 Annexin 流式细胞仪检测 体外细胞毒作用 TFAR19 剂量依赖关系 诱导细胞凋亡 细胞周期 CCK-8 共同培养 半数抑制 细胞减少 PI染色 作用机制 细胞增 G1期 

分 类 号：R284.2[医药卫生—中药学] R733.3[医药卫生—中医学]