6-BA诱导大豆子叶节和茎尖出芽的研究  

Studies on Shoots Induced by 6-BA from Cotyledonary Nodes and Embryonic Tips of Soybean

作　　者：林树柱[1] 曹越平[1] 卫志明[2] 马晓平[1] 陈鲁勇[1] 

机构地区：[1]上海交通大学农业与生物学院,上海201101 [2]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《上海交通大学学报（农业科学版）》2005年第2期138-142,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金(30370892);上海市科委发展基金(023112024)

摘　　要：以大豆品种“合丰35”的成熟种子为材料,研究6-BA诱导子叶节和茎尖出芽的结果表明,大豆萌发和不定芽诱导时分别加入0.4mg·L-1和1.1mg·L-16-BA,每个外植体分化出的芽数最多为3.56;茎尖再生系统中,6-BA诱导茎尖不定芽形成的最佳浓度是3.5mg·L-1,最佳诱导时间是24～28h左右,平均每个外植体分化出的不定芽数为2.78。Regeneration from cotyledonary nodes and embryonic tips of soybea n 'Hefeng35' was studied. In the regeneration from cotyledonary nodes, the basal m edium B5 was better in comparison with 1/2 MS B5 in adventitious shoot induction . And the optimal concentrations of 6- BA in germination medium and shoot induc tion medium were 0.4 mg· L- 1 and 1.1 mg· L- 1, and the mean of shoots per e xplant was 3.56. In the regeneration from embryonic tips, the optimal 6- BA con centration was 3.5 mg· L- 1 and the suitable inducing time of 6- BA(3.5 mg· L- 1) was about 24～ 28 h, and the mean of shoots per explant was 2.78.

关 键 词：6-BA 子叶节 茎尖 出芽 不定芽诱导 不定芽形成 成熟种子 合丰35 大豆品种 再生系统 最佳浓度 诱导时间 外植体 分化 

分 类 号：S663.1[农业科学—果树学] S565.103.5[农业科学—园艺学]