上海结缕草JD-1和结缕草属几个主要坪用草种的ISSR指纹分析  

ISSR Fingerprinting Analysis of Shanghai Zoysia DJ-1 and Several Main Species of Lawn Using Zoysia

作　　者：胡雪华[1] 何亚丽[1] 安渊[1] 陈杰[1] 赵祥强[2] 孙明 袁建康 

机构地区：[1]上海交通大学农业与生物学院,上海201101 [2]扬州大学农学院,扬州225009 [3]上海博露草坪有限公司,上海201422

出　　处：《上海交通大学学报（农业科学版）》2005年第2期163-167,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市农委重点攻关项目[农科攻字(2001)第5-20号]

摘　　要：以上海结缕草JD-1和细叶结缕草、沟叶结缕草、结缕草为试材,利用15个ISSR标记对其亲缘关系进行了分析,其中有14个ISSR标记在4个材料中获得了良好扩增,并由扩增条带构建成指纹图谱。指纹分析结果表明,上海结缕草JD-1与细叶结缕草、沟叶结缕草和结缕草3个种之间不存在亲缘上的直接联系,初步判断上海结缕草JD-1是一个新的变异材料。Shanghai Zoysia JD- 1 is a variety selected from wild germplasm o f Zoysia in Shanghai. In order to explore the relationship between Shanghai Zoys ia JD- 1 and the species of Zoysia widely cultivated in Shanghai area, ie. Zoys ia japonica, Zoysia matrella and Zoysia tenuifolia. Using fifteen ISSR markers t o identify their genetic relationship, amomg them, fourteen ISSR markers had get good amplification, and fingerprint of four Zoysia materials were created. The results obtained indicated that there are no direct genetic relationships betwee n Shanghai Zoysia JD- 1 and three lawn- using Zoysia species mentioned above. It is also suggested that Shanghai Zoysia JD- 1 is a new variety of Zoysia.

关 键 词：上海 指纹分析 结缕草属 ISSR标记 细叶结缕草 沟叶结缕草 草种 亲缘关系 指纹图谱 分析结果 变异材料 

分 类 号：S688.4[农业科学—观赏园艺] Q75[农业科学—园艺学]