柑橘体细胞杂种有性后代的创造及杂种鉴定  

CREATION AND HYBRID IDENTIFICATION OF THE SEXUAL PROGENIES USING CITRUS SOMATIC HYBRID AS POLLEN PARENT

作　　者：伊华林[1] 邓秀新[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室华中农业大学国家柑橘育种中心,武汉430070

出　　处：《实验生物学报》2005年第3期219-226,共8页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(30070079);国际科学基金(D/2998-1)

摘　　要：以异源四倍体柑橘体细胞杂种[哈姆林甜橙(Citrus sinensis Osbeck)+粗柠檬(C.jambhiri Luss)]为父本与二倍体单胚类型宜本杂4号[华农本地早橘(C.reticulata Blanco)×宜昌橙(C.ichangen- sis Swingle)]杂交,采用胚抢救技术获得了110株有性后代植株,通过染色体计数及倍性分析仪鉴定,其中93株植株为3倍体,余下的17林植株为2倍体。RAPD分析表明:获得的有性后代植株均为杂种。110 plantlets were obtained from the cross between citrus allotetraploid somatic hy- brid [Hamlin sweet orange (Citrus sinensis Osback)+Rough Lemon(C.jambhiri Luss)] (as the pollen parent) and mono-embryonic diploid type Iben No.4 [Huanongbendizao tangerine(C. reticu- lata Blanco)×Ichang papeda(C.ichangensis Swingle)] by the means of young embryo culture in vitro, and there were 93 triploids and 17 diploids through chromosome counting and the ploidy analyser identification. RAPD analyses showed that all sexual progenies were hybrid.

关 键 词：体细胞杂种 有性后代 杂种鉴定 柑橘 哈姆林甜橙 异源四倍体 染色体计数 RAPD 植株 粗柠檬 本地早 二倍体 胚抢救 分析仪 分析表 单胚 父本 倍性 

分 类 号：S666.2[农业科学—果树学]